Process for recovering useful substances from culture media

Chemistry: fertilizers – Processes and products – Forms or conditioning

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71 77, 71 79, 71 65, 195 49, 195 82, 195 66R, A01N 500

Patent

active

040185931

ABSTRACT:
A microorganism is cultivated with a liquid-containing culture medium so as to obtain a protein-containing cellular product. The latter is then recovered from the culture medium. Thereafter, the liquid portion of the culture medium is treated so as to obtain a first fraction which contains proteins and a second fraction which is substantially free of proteins and contains an active substance or active substances. The treatment of the liquid portion of the culture medium may include ultrafiltration, filtration through a molecular sieve, precipitation or solvent extraction. The active substances present in the second fraction may include growth factors and growth inhibitors for the microorganism. Certain of these growth factors and growth inhibitors are useful in the cultivation of green plants and the second fraction may be utilized for this purpose. On the other hand, all or part of the second fraction may be recycled for use in the cultivation of the microorganism. Aside from the recovery of active substances which is achieved by separating the liquid portion of the culture medium into first and second fractions, there is achieved the result that water pollution due to discharge of the culture medium may be reduced since the biochemical oxygen demand of the culture medium is decreased by removal of the proteins therefrom, that is, by recovering the protein content of the culture medium as a separate fraction.

REFERENCES:
patent: 3520777 (1970-07-01), Filosa
patent: 3551297 (1970-12-01), Hosler
patent: 3642578 (1972-02-01), Hitzman et al.
Robbins "Thiamin and Plant Growth" Science vol. 89 1939 pp. 303-307.
Micheals "New Separation Technique for the CPI" Chemical Engineering Progress vol. 64 Dec. 1968 pp. 31-43.

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