Process for detecting nucleic acid by capillary electrophoresis

Details Process for detecting nucleic acid by capillary electrophoresis Process for detecting nucleic acid by capillary electrophoresis

1994-10-04

1997-10-21

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436161, 436800, 536 266, 204452, C07H 2104, C12Q 168, G01N 2726

Patent

active

056795165

ABSTRACT:
A process for detecting a target nucleic acid, which includes the steps of hybridizing a target single-stranded nucleic acid in a sample solution to a labeled single-stranded nucleic acid probe to form a reaction mixture containing a hybrid, subjecting the reaction mixture to capillary electrophoresis to separate the hybrid from the free labeled nucleic acid probe, and detecting the hybrid utilizing the label moiety of the nucleic acid probe constituting the hybrid.

REFERENCES:
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5470705 (1995-11-01), Grossman et al.
J. Sambrook et al., "Analysis and Cloning of Eukaryotic Genomic DNA", Molecular Cloning: A Laboratory Manual, 2nd ED, published by Cold Spring Harbor Laboratory Press, 1989, pp. 9.31-9.58.
Chen et al., Journal of Chromatography 559:295-305 1991.
Vilenchik et al., Journal of Chromatography (A) 663:105-113 Mar. 4, 1994.
Bianchi et al., Journal of Virological Methods 47(3):321-329 May 1994.
Chen et al. J. Chromatography A652 (1993) 355-360.
Matthews et al. Anal. Biochem. 169:1-25, 1988.

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