Method and reagent for determination of dehydrogenase or its sub

Details Method and reagent for determination of dehydrogenase or its sub Method and reagent for determination of dehydrogenase or its sub

1991-08-15

1993-10-05

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 435 25, 435 28, 435184, 435810, 436164, 436904, C12Q 132

Patent

active

052504204

ABSTRACT:
A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to dehydrogenase reaction in the presence of an electron carrier or a combination of a coenzyme and an electron carrier, allowing to form H.sub.2 O.sub.2 through transfer of electrons derived from the substrate by the dehydrogenase reaction to O.sub.2 via the electron carrier or via the coenzyme and electron carrier, stopping the dehydrogenase reaction, reacting said H.sub.2 O.sub.2 with a peroxidase and a chromogen, and measuring a formed dye, and a reagent for determining dehydrogenase or its substrate. By the method and the reagent, dehydrogenase or its substrate can be accurately determined in a short time regardless of the concentration of the dehydrogenase and/or the substrate and without staining tubes or cells of a determination device with a dye.

REFERENCES:
patent: 3985621 (1976-10-01), Maruyama et al.
patent: 4394444 (1983-07-01), Cameron et al.
patent: 4622296 (1986-11-01), Yamanishi et al.
patent: 4629697 (1986-12-01), Limbach et al.
Chemical Abstracts, vol. 106, No. 13, p. 374 Abstract No. 99215r, Mar. 1987.

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