Zymomonas with improved xylose utilization

Details Zymomonas with improved xylose utilization Zymomonas with improved xylose utilization

2011-08-16

2011-08-16

Mondesi, Robert B (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S069100, C435S161000, C435S320100, C435S471000, C435S233000, C435S006120, C536S023200

Reexamination Certificate

active

07998722

ABSTRACT:
Strains ofZymomonaswere engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of theZ. mobilisglyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5231020 (1993-07-01), Jorgensen et al.
patent: 5514583 (1996-05-01), Picataggio et al.
patent: 5712133 (1998-01-01), Picataggio et al.
patent: 6566107 (2003-05-01), Zhang
patent: 7223575 (2007-05-01), Zhang et al.
patent: 2003/0162271 (2003-08-01), Zhang et al.
patent: 2007/0092957 (2007-04-01), Donaldson et al.
patent: 2008/0081358 (2008-04-01), Viitanen et al.
patent: 2008/0187973 (2008-08-01), Viitanen et al.
patent: 2008/0286870 (2008-11-01), Viitanen et al.
patent: 9528476 (1995-10-01), None
patent: 2004081185 (2004-09-01), None
Feldmann et al. Cloning and expression of the genes for xylose isomerase and xylulokinase fromKlebsiella pneumoniae1033 inEscherichia coliK12, Mol Gen Genet. Aug. 1992;234(2):201-10.
Schellenberg et al. Xylose isomerase fromEscherichia coli. Characterization of the protein and the structural gene, J Biol Chem. Jun. 10, 1984;259(11):6826-32.
Feldmann et al., Pentose Metabolism inZymomonas mobilisWild-Type and Recombinant Strains, Appl. Microbiol. Biotechnol., 1992, vol. 38:354-361.
Zhang et al., Metabolic Engineering of a Pentose Metabolism Pathway in EthanologenicZymomonas mobillis, Science, 1995, vol. 267:240-243.
Yanase et al., Genetic Engineering ofZympbacter palmaefor Production of Ethanol From Xylose, Appl. Environ. Microbiol., 2007, vol. 73:2592-2599.
Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, 1989, Cold Spring Harbor Laboratory: Cold Spring Harbor, New York (Book Not Included).
S. F. Altschul et al., Basic Local Alignment Search Tool. J. Mol. Biol., 1993, vol. 215:403-410.
A. M. Lesk, Computational Molecular Biology, 1988, Oxford University, NY (Book Not Included).
D. W. Smith, Biocomputing: Informatics and Genome Projects, 1993, Academic, NY (Book Not Included).
A. Griffin et al., Computer Analysis of Sequence Data, Part 1, 1994, Humania, NJ (Book Not Included).
G. Von Heinje, Sequence Analysis in Molecular Biology, 1987, Academic (Book Not Included).
M. Gribskov et al., Sequence Analysis Primer, 1991, Stockton, NY (Book Not Included).
Higgins et al., Fast and Sensitive Multiple Sequence Alignment on a Microcomputer, CABIOS, 1989, vol. 5:151-153.
D. G. Higgins et al., Clustal V: Improved Software for Multiple Sequence Alignment, Comput. Appl. Biosci., 1992, vol. 8:189-191.
W. R. Pearson, Comput. Methods Genome Res., [Proc. Int. Symp.], (1994) Meeting Date 1992, p. 111-120.
T. J. Silhavy et al., Experiments With Gene Fusions; 1984, Cold Spring Harbor Laboratory: Cold Spring Harbor, New York (Book Not Included).
F. M. Ausubel et al., Current Protocols in Molecular Biology, 1987, Greene Publishing and Wiley-Interscience (Book Not Included).
S. Tabor et al., A Bacteriophage T7 RNA Polymerase/Promoter System for Controlled Exclusive Expression of Specific Genes, Proc. Acad. Sci., 1985, vol. 82:1074-1078.
Walker et al., Isothermal In Vitro Amplification of DNA by a Restriction Enzyme/DNA Polymerase System, Proc. Natl. Acad. Sci., 1992, vol. 89:392-396.
K. E. Davis, Diagnosis of Genetic Disorders, Human Genetic Diseases: A Practical Approach, IRL Press, Herndon, VA, 1986, pp. 33-50 (Book Not Included).
W. Rychlik, Selection of Primers for Polymerase Chain Reaction, Methods in Molecular Biology, 1993, vol. 15:31-39.
Ohara et al., One-Sided Polymerase Chain Reaction: The Amplification of CDNA, PNAS, 1989, vol. 86:5673-5677.
Loh et al., Science, 1989, vol. 243:217.
Van Ness et al., The Use of Oligodeoxynucleotide Probes in Chaotrop-Based Hybridization Solutions, Nucl. Acids Res., 1991, vol. 19:5143-5151.
R. D. Finn et al., PFAM: Clans, Web Tools and Services, Nucleic Acids Research, 2006, Database Issue 34:D247-D251.
Durbin et al., Biological Sequenc Analysis: Probabilistic Models of Proteins and Nucleic Acids, 1998 (Book Not Included).
Krogh et al., Hidden Markov Models in Computational Biology, J. Mol. Biol., 1994, vol. 235:1501-1531.
Scott et al., Sequences of Versatile Broad-Host-Range Vectors of the RK2 Family, Plasmid, 2003, vol. 50:74-79.
L. R. Lynd et al., Microbial Cellulose Utilization: Fundamentals and Biotechnology, Microbiol. Mol. Biol. Rev., 2002, vol. 66:506-577.
Crueger et al., Biotechnology: A Textbook of Industiral Microbiology, Second Edition, 1989, Sinauer Associates, Inc. (Book Not Included).
Deshpande et al., Ethanol Production From Cellulose by Coupled Saccharification/Fermentation UsingSaccharomyces cerevisiaeand Cellulase Complex FromSclerotium rolfsiiUV-8 Mutant, Appl. Biochem. Biotechnol., 1992, vol. 36:227-234.
Cahoon et al., Metabolic Redesign of Vitamin E Biosynthesis in Plants for Tocotrienol Production and Increased Antioxidant Content, Nature Biotechnology, 2003, vol. 21:1082-1087.
National Center for Biotechnology Information General Identifier No. 43692, Oct. 23, 2008, M. E. Fling et al., Nucleotide Sequence of the Transposen TN7 Gene Encoding an Aminoglycoside-Modifying Enzyme, Accession No. X03043.1.
Conway et al., Glyceraldehyde-3-Phosphate Dehydrogenase Gene FromZymomonas mobilis: Cloning, Sequencing, and Identification of Promoter Region, J. Bacteriol., 1987, vol. 169:5653-5662.
U.S. Appl. No. 11/862,566, filed Sep. 27, 2007, Applicant: Paul V. Vitanen.
Frohman et al., PNAS USA, 85:8998, 1988.
Eddy, Sean R, HMMER User's Guide, Biological Sequence Analysis Using Profile Hidden Markov Models, (2010), 1-77.
Hulsen, Tim et al., Testing Statistical Significance Scores of Sequence Comparison Methods With Structure Similarity, BMC Bioinformatics (2006), 1471-2105, 7:44.
Office Action mailed Jun. 22, 2010, in co-pending U.S. Appl. No. 12/410,495.

Affiliated with

Also associated with

Rating

Zymomonas with improved xylose utilization does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Zymomonas with improved xylose utilization, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Zymomonas with improved xylose utilization will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2621976