Zymomonas pentose-sugar fermenting strains and uses thereof

Details Zymomonas pentose-sugar fermenting strains and uses thereof Zymomonas pentose-sugar fermenting strains and uses thereof

2007-05-29

2007-05-29

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S041000, C435S132000, C435S155000, C435S157000

Reexamination Certificate

active

10134655

ABSTRACT:
Disclosed in the present invention is aZymomonasintegrant and derivatives of these integrants that posses the ability to ferment pentose into ethanol. The genetic sequences encoding for the pentose-fermenting enzymes are integrated into theZymomonasin a two-integration event of homologous recombination and transposition. Each operon includes more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transketolase. TheZymomonasintegrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media. These stably integrated sequences provide a uniqueZymomonasthat may then be used for the efficient conversion of pentose sugars (xylose, arabinose) to ethanol. Method of using theZymomonasintegrants and derivatives thereof in production of ethanol from cellulosic feedstock is also disclosed. The invention also provides a method for preparing aZymomonasintegrant as part of the present invention. The hostZymomonasstrain found particularly useful in the creation of these compositions and methods isZymomonas mobilis31821.

REFERENCES:
patent: 5514583 (1996-05-01), Picataggio et al.
patent: 5712133 (1998-01-01), Picataggio et al.
patent: 5843760 (1998-12-01), Zhang et al.
patent: 2002/0151034 (2002-10-01), Zhang et al.

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