Zymogen activation peptides (ZAP) in the diagnosis of disease

Details Zymogen activation peptides (ZAP) in the diagnosis of disease Zymogen activation peptides (ZAP) in the diagnosis of disease

1991-06-26

1994-10-18

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 492, 435 44, 435188, 435810, 436518, 436536, 436548, 530327, 530329, 530330, 5303879, 5303913, 530845, G01N 33543, C12N 996, C07K 706, C07K 1400

Patent

active

053567811

ABSTRACT:
Pancreatic disease can be diagnosed by assaying a patient's body fluid such as serum or urine, for pancreatic activation peptides (PAP) released from zymogens by proteolytic activation. Particularly useful are peptides having C-terminal D.sub.4 K sequences. The method uses polyclonal or monoclonal antibodies generated and selected for C-terminal specificity on PAP so that the tests only report free PAP not parent zymogen. Also described are peptides and antibodies labelled with revealing agents and/or immobilised on solid supports and their use in diagnostic assays and kits. In pancreatic disease the tests distinguish necrotising from oedematous acute pancreatitis and permit severity prediction and monitoring as well as diagnosing chronic pancreatitis in exacerbation. Tests reporting free activation peptides from the zymogens prophospholipase A, procolipase and proelastases are also applicable in non-pancreatic diseases where activation of these zymogens, sharing sequence homology in the activation peptide C-terminal region with pancreatic isoenzymes, forms part of the molecular pathology of the condition.

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