Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-17
2000-09-26
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 2432, 536 2431, 536 2433, 935 8, 935 78, C12Q 168
Patent
active
06124094&
ABSTRACT:
The invention provides two new zoogloeal strains, mz1t and mz2t. The invention provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO:1. Examples of nucleic acids of mz1t include SEQ ID NO:2 and SEQ ID NO:3. An example of a nucleic acid of mz2t is SEQ ID NO:4. The invention also provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO:5. A method of detecting the presence of zoogloeal clusters in a wastewater sample is provided, comprising: a) contacting RNA from a sample of the wastewater with a nucleic acid comprising the nucleic acid of SEQ ID Nos:1,2,3,4 or 5 under conditions that permit specific hybridization; and b) detecting the presence of hybridization, the presence of hybridization indicating the presence of zoogloeal clusters. A new Hyphomicrobium spp. strain, designated M3, is provided herein. The invention provides novel nucleic acids of a Hyphomicrobium sp. M3. For example SEQ ID NO:6 and SEQ ID NO:7 rDNAs of M3. SEQ ID Nos:8, 9, 10, 11, 12, 13 and 14 are presumptive Hyphomicrobium spp. sequences. SEQ ID NO:15 is a consensus sequence from 6m3 and 3m3 (Hyphomicrobium sp. m3 isolated from sludge. A method of detecting the presence of a Hyphomicrobium species in a wastewater sample as described above, using a nucleic acid comprising the nucleic acid of SEQ ID NO:17 is also provided. The nucleic acids of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 can also be utilized in whole or as fragments in the detection method for Hyphomicrobium spp.
REFERENCES:
patent: 5462855 (1995-10-01), Springer et al.
Lajoie et al. "Molecular Analysis of Zoogloeal Clusters in Viscous Sludge" Proceedings of the Water Environmental Federation Annual Conference Exposition, vol. 3, 1997, pp. 317-326.
Anders et al. "Taxonomic Position of Aromatic-Degrading Denitrifying Pseudomonad Strains K 172 and KB 740 and Their Description as New Members of the Genra Thauera, as Thauera aromatica sp. nov., and Azoarcus, as Azoarcus evansii sp. nov., respectively, Members of the Beta Subclass of the Proteobacteria" Int. J. Sys. Bacteriol. 327-333, Apr. 1995.
Macy et al. "Thauera selenatis gen. Nov., sp. Nov., a Member of the Beta Subclass of proteobacteria with a Novel Type of Anaerobic Respiration" Int. J. Sys. Bacteriol., 135-142, Jan. 1993.
Fesefeldt, A. and Gliesche, C.G. "Identification of Hyphomicrobium spp. using PCR-Amplified Fragments of the mxaF Gene as a Molecular Marker" System. Appl. Microbiol. 20:387-396, 1997.
Ming-Chien, S., Cha, Dainel K., and Anderson, P.R. "Influence of Selector Technology on Heavy Metal Removal of Activated Sludge Secondary Effects of Selector Technology" Wat. Res. 29(3):971-976, 1995.
Myers, A.J. and Myers, C.D. Hyphomicrobium-mediated Sludge Bulding in an Industrial Wastewatewater Treatment System Abstract 86th Annual Meeting of the Amer. Society for Microbiol. Washington, DC Mar. 23-28, 1986, Abstracts of the Annual Meeting--1986,No. N-93, p. 257.
Rabus et al. Arch. Microbiol. (1995) 163:96-103, Apr. 1995.
Seviour, E.M. et al. (1997) "The filamentous morphotype Eikelboom Type 1863 is not a single genetic entity" J. Applied Microbiol. 82:411-421.
Reyes, F.L., Ritter, W., Raskin, L. (1997) Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems. Applied and Environmental Microbiology 63(3), 1107-1117.
Holm, N.C., Gliesche, C.G., Hirsch, P. (1996) "Diversity and structure of Hyphomicrobium populations in a sewage treatment plant and its adjacent receiving lake," Applied and Environmental Microbiology, 62(2), 522-528.
Amaral, J.A., Archambault, C., Richards, S.R., Knowles, R. (1995) Denitrification associated with Groups I and II methanotrophs in a gradient enrichment system. FEMS Microbiology Ecology 18, 289-298.
Rossello-Mora, R., Wagner, M., Amann, R., and Schleiffer, K.-H. (1995) "The abundance of Zoogloea ramigera in sewage treatment plants." Applied and Environmental Microbiology, vol. 61, No. 2, pp. #702-707.
Wagner, M., Amann, R., Kampfer, P., Assmus, B., Hartman, Hutzler, P., Springer, N., Schleifer, K. (1994) "Identification and in situ detection of gram-negative filamentous bacteria in activated sludge." Syst. Appl. Microbiol. 17, 405-417.
Jenkins, David et al. (1993) Manual on the Causes and Control of activated Sludge Bulking and Foaming, 2nd Ed., Lewis Publishers, Chelsea, Michigan.
Shin, Y.K., Hiraishi, A., and Sugiyama, J. (1993) "Molecular systematics of the genus Zoogloea and emendation of the genus." Int. J. Syst. Bacteriol., vol. 43, No. 4, pp. #826-831.
Wagner, M., Amann, R., Lemmer,H., Schleifer, K. (1993) "Probing activated sludge with oligonucleotides specific for Proteobacteria: Inadequacy of culture dependent methods for describing microbial community structure." Applied and Environmental Microbiology 59(5), 1520-1525.
Unz, R.F. (1984). "Genus IV. Zoogloea Itzigosohn." in Bergey's Manual of Systematic Bacteriology (N.R. Krieg and J.G. Holt, eds.), Williams and Wilkins, Baltimore, MD. vol. 1, pp. #214-219.
Unz, R.F. (1971). "Neotype strain of Zoogloea ramigera Itzigsohn." Int. J. Syst. Bacteriol., vol. 21, pp. #91-99.
Kelly Christine Jo
Lajoie Curtis A.
Layton Alice C.
Sayler Gary S.
Stapleton Raymond
Eastman Chemical Company
Gwinnell Harry J.
Horlick Kenneth R.
Taylor Janell E.
Tubach Cheryl J.
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