Zea mays (L.) with capability of long term, highly efficient pla

Details Zea mays (L.) with capability of long term, highly efficient pla Zea mays (L.) with capability of long term, highly efficient pla

1995-01-12

1998-06-16

Benzion, Gary

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800200, 800250, 800DIG56, 4351723, 4351721, 435424, 435430, A01H 106, A01H 400, C12N 1500, C12N 1505

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active

057673675

ABSTRACT:
Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

REFERENCES:
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