Zea mays (L.) with capability of long term, highly efficient pla

Details Zea mays (L.) with capability of long term, highly efficient pla Zea mays (L.) with capability of long term, highly efficient pla

1995-06-05

1998-08-11

Benzion, Gary

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800200, 800DIG56, 4352404, 43524049, 4352405, A01H 500, A01H 400, C12N 504

Patent

active

057929360

ABSTRACT:
Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

REFERENCES:
patent: 4975374 (1990-12-01), Goodman et al.
patent: 5220114 (1993-06-01), Martin
Morocz et al. Improved system to obtain fertile regenerates via maize protoplast isolated from a highly embryopgenic suspension culture. TAG 80:721-726, 1990.
Shillito et al. Bio/technology vol. &:581-587, 1989.
Rhodes et al. "Genetically transformed maizeplants from protoplasts" Science 40:204-207, 1986.

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