1973-11-26
1976-01-06
Monacell, A. Louis
Textiles: fiber preparation
195 78, C12B 100
Patent
active
039309460
ABSTRACT:
A process for the preparation of a stable diploid of Candida lipolytica which comprises (a) in a first stage, separately cultivating two haploid strains of Candida lipolytica of opposed sign in a medium rich in assimilable carbon nutrient; (b) in a second stage, cultivating the two resultant strains together in a medium poor in assimilable carbon nutrient; and (c) in a third stage, after the appearance of diploid colonies, treating them with a mutagenic agent to stabilize them. The invention also comprises a process for the preparation of .alpha.-ketoglutaric acid which comprises cultivating a diploid strain obtained by crossing two Candida lipolytica haploid strains of opposite sign in a medium containing, as source of assimilable carbon, at least one hydrocarbon.
REFERENCES:
patent: 3616213 (1971-10-01), Tsugawa
Binet Daniel
Desmarquest Jean-Pierre
Gaillardin Claude
Maldonado Paul
Institut Francais du Pe'trole
Institut Francaise du Petrole, des Carburants et Lubrifiants
Monacell A. Louis
Penland R. B.
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