Yeast strains for the production of mature heterologous proteins

Details Yeast strains for the production of mature heterologous proteins Yeast strains for the production of mature heterologous proteins

1995-06-07

1999-03-09

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

43525421, 435 691, 435 692, 435 697, 4353201, 536 232, 536 234, 536 235, 536 242, C12N 119, C12N 1515, C12N 1512, C07H 2104

Patent

active

058799265

ABSTRACT:
The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.

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