Yeast silencing genes proteins and methods

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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43525421, 4353201, 536 2374, C12N 119, C12N 118, C12N 1531, C12N 1563

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059898976

ABSTRACT:
The present invention provides the yeast genes SAS2, SAS3 and ESA1 and the proteins encoded thereby. SAS2, SAS3 and ESA1 genes of members of the genus Saccharomyces are provided, particularly the SAS2, SAS3 and ESA1 genes of S. cerevisiae. Also provided are yeast SAS2, SAS3 and ESA1 coding sequences. Specifically provided are the SAS2, SAS3 and ESA1 coding sequences of members of the genus Saccharomyces, and more specifically of S. cerevisiae. Genes of this invention comprise protein coding sequences as well as the regulatory regions that control expression of the encoded protein. Of most interest are SAS2, SAS3, and ESA1 genes of yeast including those of the genus Saccharomyces which are 90% or more homologous to the corresponding genes of S. cerevisiae. Specifically provided are DNA constructs comprising purified and isolated DNA molecules comprising SAS2, SAS3 or ESA1 coding sequences that encode proteins from a strain of S. cerevisiae.

REFERENCES:
Abraham, J., et al. "Regulation of Mating-type Information in Yeast" J. Mol. Biol., 176:307-331 (1984).
Aparicio, O.M., et al. "Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae," Cell, 66:1279-1287 (1991).
Axelrod, A. and Rine, J. "A role for CDC7 in repression of transcription at the silent mating-type locus HMR in Saccharomyces cerevisiae," Mol. and Cell. Biol., 11(2):1080-1091 (1991).
Bassett Jr., D.E., et al. "Yeast genes and human disease," Nature, 379:589-590 (1996).
Baudin, A., et al. "A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae," Nucleic Acids Research, 21(14):3329-3330 (1993).
Borrow, J., et al. "The translocation t(8;16)(p11;p13) of acute myeloid leukaemia fuses a putative acetyltransferase to the CREB-binding protein," Nature Genetics, 14:33-41 (1996).
Brachmann, C.B., et al. "The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability," Genes & Dev., 9:2888-2902 (1995).
Brand, A.H., et al. "A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation," Cell, 51:709-719 (1987).
Braunstein, M., et al. "Transcriptional silencing in yeast is associated with reduced nucleosome acetylation," Genes & Dev., 7:592-604 (1993).
Brownell, J.E. and Allis, C.D. "Special HATs for special occasions: linking histone acetylation to chromatin assembly and gene activation," Curr. Opin. in Genes & Devl, 6:176-184 (1996).
Brownell, J.E. and Allis, C.D. "An activity gel assay detects a single, catalytically active histion acetyltransferase subunits in Tetrahymena macronuclei," Proc. Natl. Acad. Sci. USA, 92:6364-6368 (1995).
Brownell, J.E., et al. "Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation," Cell, 84:843-851 (1996).
Chrivia, J.C., et al. "Phosphorylated CREB binds specifically to the nuclear protein CBP," Nature, 365:855-859 (1993).
Dillin, A. and Rine, J. "On the origin of a silencer," TIBS, 20:231-235 (1995).
Feldman, J., et al. "Identification of Sites Required for Repression of a Silent Mating Type Locus in Yeast" J. Mol. Biol., 178:815-834 (1984).
Gray, T.E., et al. "Refolding of barnase mutants and pro-barnase in the presence and absence of GroEL," EMBO J., 12(11):4145-4150 (1993).
Greenwell, P.W., et al. "TEL1, a gene involved in controlling telomere length in S. cerevisiae, is homologous to the human ataxia telangiectasia gene," Cell, 82:823-829 (1995).
Gottschling, D.E., et al. "Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription," Cell, 63:751-762 (1990).
Hawthorne, D.C. "A deletion in yeast and its bearing on the structure of the mating type locus," Genetics, 48:1727-1729 (1963).
Hebbes, T.R., et al. "A direct link between core histone acetylation and transcriptionally active chromatin," EMBO J., 7(5):1395-1402 (1988).
Hong, L., et al. "Studies of the DNA binding properties of histone H4 amino terminus," J. Biol. Chem., 268(1):305-314 (1993).
Johnson, L.M., et al. "Genetic evidence for an interaction between SIR3 and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae," Proc. Natl. Acad. Sci. USA, 87:6286-6290 (1990).
Kamine, J., et al. "Identification of a cellular protein that specifically interacts with the essential cysteine region of the HIV-1 tat transactivator," Virology, 216:357-366 (1996).
Kassir, Y., et al. "SAD Mutation of Saccharomyces cerevisiae Is an Extra a Cassette"Mol. Cell. Biol, 3:871-880 (1983).
Kayne, P.S., et al. "Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast," Cell, 55:27-39 (1988).
Kennedy, B.K., et al. "Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae," Cell, 80:485-496 (1995).
Kimmerly, W., et al. "Roles of two DNA-binding factors in replication, segregation and transcriptional represion mediated by a yeast silencer" EMBO J., 7:2241-2253 (1988).
Kleff, S., et al. "Identification of a gene encoding a yeast histone H4 acetyltransferase," J. Biol. Chem., 270(42):24674-24677 (1995).
Lee, D.Y., et al. "A positive role for histone acetylation in trnascription factor access to nucleosomal DNA," Cell, 72:73-84 (1993).
Lee, F-J., et al. "Acetylation is required for normal growth and mating of Saccharomyces cerevisiae," J. Bacteriol., 171(11):5795-5802 (1989).
Loo, S. and Rine, J. "Silencing and heritable domains of gene expression," Annu. Rev. Cell Dev. Biol., 11:519-548 (1995).
Mahoney, D.J., et al. "Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression," Genes & Dev., 5:605-615 (1991).
Marcus, G.A., et al. "Functional similarity and physical association between GCN5 and ADA2: putative transcriptional adaptors," EMBO J., 13(20):4807-4815 (1994).
Megee, P.C., et al. "Genetic analysis of histone H4: essential role of lysines subject to reversible acetylation," Science, 247:841-845 (1990).
Morrow, D.M., et al. "TEL1, and S. cerevisiae homolog of the human gene mutated ataxia telangiectasia, is functionally related to the yeast checkpoint gene MEC1," Cell, 82:831-840 (1995).
Mullen, J.R., et al. "Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast," EMBO J, 8(7):2067-2075 (1989).
Ehrenhofer-Murray, A.E., et al. "The role of Sas2, an acetyltransferase homologue of Saccharomyces cerevisiae, in silencing and ORC function," Genetics, 145:923-934 (1997).
Park, E-C. and Szostak, J.W. "Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML," Mol. Cell. Biol., 10(9):4932-4934 (1990).
Parthun, M.R., et al. "The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism," Cell, 87:85-94 (1996).
Pennisi, E. "Opening the way to gene activity," Science, 275:155-157 (1997).
Pillus, L. and Grunstein, M. "Chromatin structure and epigenetic regulation in yeast" Chromatin Structure and Gene Expression (ed. Elgin, S.C.R.) IRL Press, Oxford University Press, pp. 123-146 (1995).
Pillus, L. and Rine, J. "Epigenetic inheritance of transcriptional states in S. cerevisiae," Cell, 59:637-647 (1989).
Reifsnyder, C., et al. "Yeast SAS silencing genes and human genes associated with AML and HIV-1 Tat interactions are homologous with acetyltransferases," Nature Genetics, 14:42-49 (1996).
Renauld, H., et al. "Silent domains are assembled continously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage," Genes & Dev., 7:1133-1145 (1993).
Roth, S.Y. and Allis, C.D. "Histone acetylation and chromatin assembly: a single escort, multiple dances?" 87:5-8 (1996).
Roth, S.Y. "Something about silencing," Nature Genetics, 14:3-4 (1996).
Rothstein, R. "Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast," Meth. Enzymol., 194:281-301 (1991).
Savitsky, K, et al. "A single ataxia telangiectasia gene with a product similar to

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