Yeast shuttle vector

Details Yeast shuttle vector Yeast shuttle vector

1986-12-23

1991-03-05

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 91, 435171, 4351721, 4351723, 435911, 435940, 435942, 536 27, 935 6, 935 8, 935 9, 935 22, 935 23, 935 24, 935 27, 935 28, 935 33, 935 36, 935 37, 935 59, C12N 1579, C12N 1581, C12N 1509

Patent

active

049977679

ABSTRACT:
Shuttle vectors including a DNA sequence of the yeast Saccharomyces cerevisiae including ars 1.2 micron ori and a marker gene for transformed yeast permitting synthesis of leucine by the transformant; adjacent to said ars 1 a DNA sequence of Escherichia coli which is either in EcoR1--PvuII or EcoR1--TthIII--I fragment of plasmid pBR322; and adjacent to the yeast marker gene the expression control region of the repressible acid phosphatase gene of yeast.

REFERENCES:
Maniatis et al, 1982, Molecular Cloning:A Laboratory Manual, Cold Spring bor Laboratory.
Miyanohara et al, 1983, PNAS 80:1-5.
Kramer et al, 1984, PNAS 81:367.
Ronald A. Hitzeman et al., Expression of a Human Gene for Interferon in Yeast, Nature, vol. 293, pp. 717-722, (1981).
B. Meyhack et al., Two Yeast Acid Phosphatase Structural Genes are the Result of a Tandem Duplication and Show Different Degrees of Homology in their Promoter and Coding Sequences, The Embo Journal, vol. 1, No. 6, 675-680 (1982).
Jill Ferguson et al., Gene, vol. 16, pp. 191-197, (1981).
Hans Rudolph et al., Proc. Natl. Acad. Sci. USA, vol. 84, pp. 1340-1344 (1987).
Kenji Arima, et al., The Nucleotide Sequence of the Yeast PHO5 Gene: A Putative Precursor of Repressible Acid Phosphatase Contains a Signal Peptide, Nucleic Acids.
Research, vol. 11, No. 6, pp. 1657-1672, (1983).

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