Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-08-24
1991-06-04
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 91, 4351723, 4353201, 435255, 435256, 536 27, 935 37, C07H 1512, C12N 100, C12P 1931
Patent
active
050213393
ABSTRACT:
A DNA sequence of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) promoter and a process for preparing a heterologous protein utilizing said DNA sequence as a promoter are disclosed, said GAP-DH promoter comprising a region upstream of an initiator codon of the GAP-DH protein up to -164 bp as a minimum unit. Since the GAP-DH promoter is small in size, a physiologically active substance can be expressed in yeasts effectively and recombination of DNA can be simplified.
REFERENCES:
patent: 4618578 (1986-10-01), Burke et al.
patent: 4722840 (1988-02-01), Valenzuela et al.
Bitter, G. A. et al., Expression of Heterologous Genes in S. cerevisiae from Vectors Utilizing the Glyceraldehyde-3-Phosphate Dehydrogenase Gene Promoter; Gene 32 (1984), pp. 263-274.
Volker, T. A. et al; Deletion Analysis of a Bacteriophage T.sub.4 Late Promoter; Gene 33 (1984), pp. 207-213.
Holland, G. P. et al; Homologous Nucleotide Sequences at the 5' Termini of Messenger RNAs Synthesized from the Yeast Enolase and Glyceradehyde-3-Phosphate Dehydrogenese Gene Families; J. Biol. Chem. 258 (1983), pp. 5291-5299.
Cell, vol. 37, pp. 629-633, Feb. 1984.
Arimura Hirofumi
Horii Hajime
Kawabe Haruhide
Mukai Hiromichi
Suyama Tadakazu
Green Cross Corporation
Teskin Robin L.
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