Yeast processing system

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, C12P 2100

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active

055102492

ABSTRACT:
A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof.

REFERENCES:
Thim et al., Proc. Natl. Acad. Sci. USA, vol. 83, pp. 6766-6770 (1986).
Brake et al., Proc. Natl. Acad. Sci. USA, vol. 81, pp. 4642-4646 (1984).

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