Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-03-02
1996-04-23
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, C12P 2100
Patent
active
055102492
ABSTRACT:
A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof.
REFERENCES:
Thim et al., Proc. Natl. Acad. Sci. USA, vol. 83, pp. 6766-6770 (1986).
Brake et al., Proc. Natl. Acad. Sci. USA, vol. 81, pp. 4642-4646 (1984).
Bj.o slashed.rn S.o slashed.ren
Norris Fanny
Norris Kjeld
Agris Cheryl H.
Novo Nordisk A S
Patterson Jr. Charles L.
Zelson Steve T.
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