Yeast processing system

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530303, 435 691, 435 697, C07K 1300, C07K 740, C12P 2102

Patent

active

053959221

ABSTRACT:
A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof.

REFERENCES:
Zsebo, K. M., et al. (1986) J. Biol. Chem. 261 (13), 5858-5865.
Kurjan, J., et al. (1982) Cell 30, 933-943.
Biological Abstract, 12824.
Thim et al., Proc. Natl. Acad. Sci. USA, vol. 83, pp. 6766-6770 (1986).

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