Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Patent
1992-07-31
1994-06-28
Knode, Marian C.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
435942, C12N 116
Patent
active
053246594
ABSTRACT:
Disclosed is a process utilizing yeast mutants for identifying active FK-506 type immunosuppressants. The process utilizes Saccharomyces cerevisiae mutants containing an fkr1, fkr2, fkr3 mutant gene or mixture thereof, which are resistant to FK-506 but sensitive to rapamycin. These mutants can be used in a diagnostic procedure for identifying FK-506 and FK-506 type immunosuppressants and in screening assays for compounds and fermentation broths which exhibit FK-506 type immunosuppressive activity. Specifically disclosed are the new yeast mutants, Saccharomyces cerevisiae YKF093, (Merck Culture Collection No. MY 2088) ATCC No. 74055, containing an fkr3 mutant gene, Saccharomyces cerevisiae YKF 012, (Merck Culture Collection No. MY 2096) ATCC No. 74061, which contains an fkr1 mutant gene, Saccharomyces cerevisiae YFK 014, (Merck Culture Collection No. MY 2097) ATCC No. 74062 and YFK-023-17A (Merck Culture Collection No. MY 2098) ATCC No. 74063, both which contain an fkr2 mutant gene and are useful in identifying active FK-506 type immunosuppressants.
REFERENCES:
Mol. & Collular Biol., vol. 11, No. 9, pp. 4616-4626 (Sep. 1991), by L. Brizuela, et al., entitled Antifungal Properties of the Immunosuppressant FK-506: Identification of an FK-506-Responsive Yeast Gene Distinct from FKB1.
Transplantation, vol. 47, No. 2, pp. 356-359 (Feb. 1989), by N. Yoshimura, et al., entitled Effect of a New Immunosuppressive Agent, FK506, on Human Lymphocyte Responses In Vitro.
Proc. Natl. Acad. Sci. USA, vol. 88, pp. 1948-1952 (1991) by Heitman, et al. (Cumulative).
Mol. & Cell. Biology (3/91), pp. 1718-1723 by Koltin, et al. (Cumulative).
J. Biol. Chem. 262:16871-16879 (1987) by Koltin, et al. (Cumulative).
Proc. Natl. Acad. Sci. USA 87:9231-9235 (1990), by Bierer, et al. (Cumulative).
Anal. Bioch. 72:248-254 (1976) by M. Bradford (Cumulative).
Eur. J. Appl. Microbiol. 3:125-133 (1976) by Dreyfuss, et al. (Cumulative).
J. Immunol. 144:251-258 (1990), by F. Dumont, et al. (Cumulative).
J. Immunol. 144:1418-1424 (1990), by F. Dumont, et al. (Cumulative).
Bio. Research on Indus. Yeasts, vol. III CRC Press, pp. 99-104 (1986), by Falco, et al. (Cumulative).
Can J. Chem. 58, 579-590 (1980) by Findlay, et al. (Cumulative).
Nature 337:476-478 (1989) by Fischer, et al. (Cumulative).
Gene 83:39-46 (1989), by Haendler, et al. (Cumulative).
Science 226:544-547 (1984), by Handschumacher, et al. (Cumulative).
Nature 341:758-760 (1989), by Harding, et al. (Cumulative).
J. Lab. Clin. Med. 72:511-516 (1968) by Harwick, et al. (Cumulative).
J. Antibiotics 40:1249-1255 (1987) by Kino, et al. (Cumulative).
J. Antibiotics 40:1256-1265 (1987) by Kino, et al. (Cumulative).
J. Immunol. 137:1054-1059 (1986) by Koletsky, et al. (Cumulative).
Proc. Natl. Acad. Sci. USA 81:5214-5218 (1984), by Kronke, et al. (Cumulative).
Nature 329:268-270 (1987), by Lang, et al. (Cumulative).
Genetics 125:13-20 (1990), by Leppert, et al. (Cumulative).
Can. J. Physiol. Pharmacol. 55:48-51 (1977), by Martel, et al. (Cumulative).
Transplantation 49:798-802 (1990) by Metcalfe, et al. (Cumulative).
Enzymology, vol. 101 pp. 202-211 (1983), by Rothstein (Cumulative).
Can. J. Genet. Cytol. 24:493-503 (1982) by Saunders, et al. (Cumulative).
J. Immunol. 139:1797-1803 (1987) by Sawada, et al. (Cumulative).
Proc. Natl. Acad. Sci. USA 86:5390-5394 (1989) by Schneuwly, et al. (Cumulative).
Lab. Course Manual for Methods in Yeast Genetics (1986) by Sherman, et al. (Cumulative).
Ann. Rev. Immunol., vol. 3, pp. 397-423 (1985), by Shevach (Cumulative).
Nature 338:67-70 (1989), by Shieh, et al. (Cumulative).
J. Immunol. 143:1580-1583 (1989), by Siekierka, et al. (Cumulative).
Nature 341:755-757 (1989), by Siekierka, et al. (Cumulative).
J. Biol. Chem. 265:21011-21015 (1990), by Siekierka, et al. (Cumulative).
Bioch. Pharmacol, 40:2201-2208 (1990) by Sigal, et al. (Cumulative).
Genetics 122:19-27 (1989), by Sikorski, et al. (Cumulative).
J. Mol. Biol. 153:305-321 (1981), by Sprague, Jr., et al. (Cumulative).
Lancet 2:1000-1004 (1989) by Starzl, et al. (Cumulative).
Curr. Gent. 10:665-670 (1986) by Subid, et al. (Cumulative).
Nature 337:473-475 (1989) by Takahashi, et al. (Cumulative).
Transp. Proceed. 19:11-16 (1987) by Tanaka, et al. (Cumulative).
Immunol. Today 10:6-9 (1989) by Thomson (Cumulative).
Immunol. Today 11:35-36 (1990) Thomson (Cumulative).
J. Immunol. 143:718-726 (1989), by Tocci, et al. (Cumulative).
J. Biol. Chem. 263:14433-14440 (1988), by Tropschug, et al. (Cumulative).
Nature 342:953-955 (1989) by Tropschug, et al. (Cumulative).
Nature 346:674-677 (1990), by Tropschug, et al. (Cumulative).
J. Antibiot. 28:721-726 (1975), by Vezina, et al. (Cumulative).
Proc. Natl. Acad. Sci. USA (1991), by Wiederrecht, et al. (Cumulative).
Transplantation 47:356-359 (1989), by Yoshimura, et al. (Cumulative).
Mol. and Cell. Biol., Sep. 1991, pp. 4616-4626, by Brizuela, et al. (Cumulative).
Bostian Keith A.
Brizuela Leonardo E.
Chrebet Gary L.
Parent Stephen A.
Caruso Charles M.
Knode Marian C.
Merck & Co. , Inc.
North Robert J.
Thies J. Eric
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