Yeast host strains with defects in N-glycosylation

Details Yeast host strains with defects in N-glycosylation Yeast host strains with defects in N-glycosylation

1996-05-31

1998-08-25

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435171, 4351721, 4351723, 4352552, 43525521, C12N 119, C12N 118, C12N 1567, C12P 2100

Patent

active

057982260

ABSTRACT:
Saccharomyces mutants with defects in N-glycosylation which are obtainable selective markers, selection of those strains which, after transformation with the plasmid YEpL/glucose oxidase, secrete 10 mg/l glucose oxidase or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.

REFERENCES:
Hitzeman et al., Nucl. Acids Res., 11:2745-2762 (1983).
Wood et al., Nature, 314:446-449 (1985).
Green et al., J. Biol. Chem., 261(16):7558-7565 (1986).
Nagasu et al., "Isolation of New Temperature-Sensitive Mutants of Saccharomyces cerevisae Deficient in Mannose Outer Chain Elongation", Yeast, vol. 8: 535-547 (1992).
Nakayama et al., "OCH1 encodes a novel membrane bound mannosyl-transferase: outer chain elongation of asparagine-linked oligo-saccharides", EMBO Journal, vol. 11, No. 7, pp. 2511-2519, (1992).
Kopetzki et al. "Enzymes in Diagnostics:Achievements and Possibilities of Recombinant DNA Technology", Clin. Chem 40/5: 688-704, (1994).
Huffaker et al. Temperature Sensitive Yeast Mutants Deficient in Asparagine-linked Glycosylation, The Journal of Biological Chem., vol. 257, No. 6, Mar. 25, pp. 3203-3210, 1982.
Hirschberg et al., Selection of Mutant Chinese Hamster Ovary Cells Altered in Glycoproteins by Means of Tritiated Fucose Suicide, Molecular and Cellular Biology, Oct. 1981, pp. 02-909.
Bekkers, A.C.A.P.A; Franken, P.A.F.; Van den Bergh, C.J.; Verbakel, J.M.A.; Verheij, H.M.; De Haas, G.H.: The use of genetic engineering to obtain efficient production of porcine pancreatic phospholipase A2 by Saccharomyces cerevisiae. Biochim. Biophys. Acta 1089, 345-351 (1991).
Ballou, L.; Cohen, R.E.; Ballou, C.E.: Saccharomyces cervisiae mutants that make mannoproteins with a truncated carbohydrate outer chain. J. Biol. Chem. 255, 5986-2991 (1980).
Ballou, C.E.: Yeast cell wall and cell surface. In: Strathern, J.N.; Jones, E.W.; Broach, J.R. (eds.), The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression, Cold Spring Harbor Laboratory, New York, pp. 335-360 (1982).
Ballour, L.; Alvardo, E.; Tsai, P.; Bell, A; Ballou, C.E.: Protein glycosylation defects in the Saccharomyces cerevisiae mnn7 mutant class. J. Biol. Chem. 264, 11857-11864 (1989).
Ballou, C.E.: Isolation characterization, and properties of Saccharomyces cerevisiae mmn mutants with nonconditional protein glycosylation defects. Methods Enzymol. 185, 440-470 (1990).
Ballou, L.; Hitzeman, R.A.; Lewis, M.S.; Ballou, C.S.: Vanadate-resistant yeast mutants are defective in protein glycosylation. Proc. Natl. Acad. Sci. 88, 3209-3212. (1991).
Beggs, J.D.: Transformation of yeast by a replicating hybrid plasmid. Nature 275, 104-109 (1978).
Carlson, M.; Taussig, R.; Kustu, S.; Botstein, D.: The secreted form of invertase in saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence. Mol. Cell. Biol. 3, 439-447 (1983).
Ciriacy, M.: Genetics of alcohol dehyrdogenase in Saccharomyces cerevisiae. I. Isolation and genetic analysis of ad-mutants. Mut. Res. 29, 315-326 (1975).
De Baetselier, A.; Vasavada, A.; Dohet, P.; Ha-Ti, V.; De Beukelaer, M.; M.; Erpicum, T.; De Clerk, L.; Hanotier, J.; Rosenberg, S.: Fermentation of yeast producing A. niger glucose oxidase: scale up, purification and characterization of the recombinant enzyme. Biotechnology 9, 559-561 (1991).
Delorme, E.: Transformation of saccharomyces cerevisiae by electroporation. Applied and Environmental Microbiology 55, 2242-2246 (1989).
Dijken, J.P. van; Veenhuis M.: Cytochemical localization of glucose oxidase in peroxisomes of Aspergillus niger. European J. Appln. Microbiol. Biotechnol. 9, 275-283 (1980).
Erhart, E.; Hollenberg, C.P.: The presence of a defective LEU2 gene on 2uDNA recombinant plasmids of saccharomyces cerevisiae is responsible for curing and and high copy number. J. Bacteriol. 156, 625-635 (1983).
Frederick, K.R.; Tung, J.: Emerick, R.S.; Masiarz, F.R.; Chamberlain, S.H.; Vasavada, A.; Rosenberg, S.; Chakraborty, S.; Schopter, L.M.; Massey, N.: Glucose oxidase from Aspergillus niger. J. Biol. Chem. 265, 3793-3802 (1990).
Hadwick, K.G.; Lewis, M.j.; Semenza, J.; Dean, N.; Pelham, H.R.B.: ERD1, a yeast gene required for the retention of luminal endoplasmic reticulum proteins, affects glycoprotein processing in the Golgi apparatus. EMBO J. 9, 623-630 (1990).
Haselbeck, A.; Hosel, W.: Studies on the effect of the incubation conditions, various detergents and protein concentration on the enzymatic activity of N-glycosidase F (glycopeptidase F) and endoglycosidase F. Topics in Biochemistry 8, 1-4 (1988).
Hochuli, E.; Doebeli, H.; Schacher, A.: New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J. Chromatography 411, 177-184 (1987).
Hochuli, E.; Bannworth, W.; Doebeli, H.; Genz, R.; Stueber, D.: Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Biotechnology 6, 1321-1325 (1988).
Huffaker, T.C.; Robbins, P.W.: Yeast mutants deficient in protein glycosylation. Proc. Natl. Acad. Sci. 80, 7466-7470 (1983).
Innis, M.A.: Glycosylation of heterologous proteins in saccharomyces cerevisiae. In: Barr, P.J.; Brake, A.J.; Valenzuela, P. (eds.), Yeast genetic engineering, Butterworths, Stoneham, Mass, pp. 233-246 (1989).
Ito, H.; Jukuda, A.; Murata, K.; Kimura, A: Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168 (1983).
Kopetzki, E.; Buckel, P.; Schumacher, G.: Cloning and characterization of baker's yeast alpha glucosidase: overexpression in a yeast strain devoid of vacuolar proteinases. Yeast 5, 11-24 (1989).
Kornfeld, R.; Kornfeld, S.: Assembly of asparagine-linked oligosaccharides. Ann. Rev. Biochem. 54, 631-664 (1985).
Kukuruzinska, M.A.; Bergh, M.L.E.; Jackson, B.J.: Protein glycosylation in yeast. Ann. Rev. Biochem. 56, 915-944 (1987).
Kriechbaum, M.; Heilmann, H,J.; Wientjes, F.J; Han, M.; Jany, K.-D.; Gassen, H.G.; Sharif, F.; Alaeddinoglu, G.: Clonig and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3. FEBS Lett. 255, 63-66 (1989).
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
Maniatis, T. et al., In: Molecular cloning: A laboratory manual. Cold Springs Harbor Laboratory Press, Cold Spring Harbor, New York (1989).
Moir, D.T.: Yeast mutants with increased secretion efficiency. In: Barr, P.J.; Brake, A.J.; Valenzuela, P. (eds.), Yeast genetic engineering, Butterworths, Stoneham, Mass, pp. 215-231 (1989).
Mullis, K.B.; Faloona, F.A.: Specific synthesis of DNA in vitro via a poly-merase-catalyzed chain reaction. Methods Enzymol. 155, 335-360 (19870.
Nakano, A.; Muramatsu, M.: A novel GTP-binding protein, Sarpl, is involved in transport form the endoplasmic reticulum to the Golgi apparatus. J. Cell. Biol. 109, 2677-2691 (1989).
Newman, A.P.; Ferro-Novick, S.: Characterization of new mutants in the selection. J. Cell. Biol. 105, 1587-1594 (1987).
Novick, P.; Field, C.; Schekman, R.: Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21, 205-215 (1980).
Paulson, C.P.: Glycoproteins: what are the sugar chains for? TIBS 14, 272-276 (1989).
Rudolph, H.K., Antebi, A.; Fink, G.R.; buckley, C.M.; Dorman, T.E.; LeVitre, J.; Davidow, L.S.; Mao, J.; Moir, D.T.: The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family. Cell 58, 133-145 (1989).
Reddy, V.A.; Johnson, R.S.; Biemann, K.; Williams, R.S.; Ziegler, F.D.; Trimble, R.B.; Maley, F.: Characterization of glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons. J. Biol. Chem. 263, 6978-6985 (1988).
Runge, K.W.; Robbins, P.W.: Saccharomyces cerevisiae mutants in the early stages of protein glycosylation. In: Bonventre, P.F.; Morello, J.A.M.; Silver, S.D.; Wu, H.C. (eds), Microbiology--1986. American

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