Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
C435S325000, C536S023100, C530S350000
The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast α factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.
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Broach James R.
Fowlkes Dana M.
Klein Christine A.
Manfredi John P.
Murphy Andrew J. M.
Cadus Technologies, Inc.
Carlson Karen Cochrane
Edwards Angell Palmer & & Dodge LLP
Lauro, Esq. Peter C.
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