Yeast bacterial two-hybrid system and methods of use thereof

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S320100, C435S252300, C435S254200

Reexamination Certificate

active

07919610

ABSTRACT:
A combined yeast/bacterial two-hybrid system is disclosed.

REFERENCES:
Tolmachov, O. Designing plasmid vectors. Methods Mol Biol. 2009;542:117-29.
Fields, et al. “A novel genetic system to detect protein-protein interactions.” Nature, 340(6230): 245-246 (Jul. 20, 1989).
Joung, et al. “A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions.” Proc. Natl. Acad. Sci. USA, 97(13): 7382-7387 (Jun. 20, 2000).
Nagashima, K., et al. “Structure of the two genes coding for polypeptide chain elongation factor 1α(EF-1α) fromSaccharomyces cerevisiae.” Gene, 45: 265-273 (1986).
De Boer, H. A., et al. “The tac promoter: A functional hybrid derived from the trp and lac promoters.” Proc. Natl. Acad. Sci. USA, 80: 21-25 (Jan. 1983).
Serebriiskii, I. G., et al. “Two-Hybrid System for Characterization of Protein-Protein Interactions inE. coli.” BioTechniques, 29: 288-296 (Aug. 2000).
Serebriiskii, I. G., et al. “A Combined Yeast/Bacteria Two-hybrid System.” Molecular & Cellular Proteomics, 4(6): 819-826 (Jan. 1, 2005).

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