Yeast-Bacteria shuttle vector

Details Yeast-Bacteria shuttle vector Yeast-Bacteria shuttle vector

2005-05-24

2005-05-24

Falk, Anne-Marie (Department: 1636)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

C435S254200, C800S025000

Reexamination Certificate

active

06897353

ABSTRACT:
The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described, that can be used to clone large regions of DNA by homologous recombination. The important feature of present invention is the presence of the a bacterial replication origin, which allows large DNA insert capacity. The utility of this vector lies in its ability to isolate, manipulate and maintain large fragments in bacteria and yeast, allowing for mutagenesis by yeast genetics and simplified preparation of plasmid DNA in bacteria.

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