Yeast-bacteria shuttle vector

Details Yeast-bacteria shuttle vector Yeast-bacteria shuttle vector

1996-12-06

1999-02-02

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352523, 4352542, 43525421, 4353201, C12N 115, C12N 121, C12N 1570, C12N 1581

Patent

active

058664041

ABSTRACT:
The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described, that can be used to clone large regions of DNA by homologous recombination. The important feature of present invention is the presence of the a bacterial replication origin, which allows large DNA insert capacity. The utility of this vector lies in its ability to isolate, manipulate and maintain large fragments in bacteria and yeast, allowing for mutagenesis by yeast genetics and simplified preparation of plasmid DNA in bacteria.

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