Y chromosome specific nucleic acid probe and method for identify

Details Y chromosome specific nucleic acid probe and method for identify Y chromosome specific nucleic acid probe and method for identify

1995-10-06

1999-03-30

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, C12Q 168

Patent

active

058887300

ABSTRACT:
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.

REFERENCES:
patent: 5055393 (1991-10-01), Kwoh et al.
patent: 5683195 (1997-11-01), Mullis et al.
Wallace et al., Methods Enzymol. 152:432-442, 1987.
Nakahori et al., Nuc. Acids Res. 14(19):7569-7580, 1986.
Goonewardena et al., Amer. J. of Human Genet. 45(4):A190, Oct. 1989.

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