Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1994-11-03
1999-02-02
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
4351723, 435155, 435157, C12P 702, C12P 718, C12P 1902, C12N 1552
Patent
active
058663827
ABSTRACT:
This invention relates to recombinant-DNA-technology. Specifically, this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.
REFERENCES:
patent: 4894338 (1990-01-01), Knowles et al.
Hallbom et al. (1991) "Xylitol production by recombinant S. cerevisiae" Bio/Technology 9:1090-1095.
Meinander et al (1994) Fed-batch xylitol production with recombinant XYL-1 expressing S:cerevisiae using ethanol as a co-substrate Appl. Microbiol. Biotechnol. 42: 334-339.
Koetter et al (1993) "Yylose fermentation by S. cerevisiae" Appl. Microbiol. Biotechnol. 38:776-783.
Young et al. (1983) Science vol. 222 pp. 778-782.
McKnight (1983) PNAS vol. 80 pp. 4412-4416
Winnacker (1987) From Genes to Clones, Weinheim.
J. Hagedorn et al, Isolation and Characterization of xyl mutants in a xylose-utilizing yeast, Pichia stipitis, Curr Genet 16 : 27-33 (1989).
R. Amore et al, The fermentation of Xylose- an analysis of the expression of Bacillus and Actinoplanes xylose isomerase genes in yeast, Appl. Microbiol Biotechnol 30:351-357 (1989).
M. Rizzi et al, Xylose fermentation by yeasts: Purification and kinetic studies of xylose reductase from Pichia stipitis, Appl. Microbiol. Biotechnol 29: 148-154 (1988).
M. Rizzi et al, Purification and Properties of the NAD + - Xylitol-Dehydrogenase from the Yeast Pichia stipitis, Journal of Fermentation and Bioengineering, 67: 20-24 (1989).
P. Kotter et al, Isolation and Characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant, Curr Genet 18: 493-500 (1990).
P. Bolen et al, Identification of a cDNA clone encoding aldose-reductase from the xylose-fermenting yeast Pachysolen tannophilus--ethanol production (conference abstract), Abstr. Annu. Meet. Am. Soc. Microbiol. (86 Meet., 262 0-7) (1986).
Bolen et al, Journal of Fermentation and Bioengineering, vol. 69, No. 4, pp. 211-214 (1990).
VanCauwenberge et al, Enzyme Microb. Technol., vol. 11, pp. 662-667 (Oct. 1989).
Bolen et al, Applied and Environmental Microbiology, vol. 52, No. 4, pp. 660-664 (Oct. 1986).
Bolen et al, Biotechnology and Bioengineering Symp., 15, pp. 129-148 (1985).
Bolen et al, Biotechnology and Bioengineering, vol. XXVII, pp. 302-307 (1985).
Smiley et al, Biotechnol. Lett., vol. 4, pp. 601-610 (1982)--Abstract only.
Kotter et al. "Expression of the Pichia Stipitis . . . " Yeast 1990. vol. 6, Spec. Issue, s. S604, Jun.
Yang et al. "Purification and Properties of Xylitol", Applied Biochem. and Biotech. 1990, pp. 197-206.
Prior et al. "Fermentation of D-Xylose . . . ", Process Biochem. Feb., 1989, pp. 21-32.
Ho, et al. "Purification, Characterization, and . . . " Enzyme Microb. Technol., 1990, vol. 12, Jan., pp. 33-39.
Beach et al., Nature, 290, 140-142 (1981).
Das et al., Current Genetics 6:123-128 (1982).
Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Ed., 1989, pp. 8.46-8.53.
Airaksinen Ulla
Hahn-Hagerdal Barbel
Hallborn Johan
Keranen Sirkka
Ojamo Heikki
Chambers Jasemine C.
Priebe Scott D.
Xyrofin Oy
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