Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-08-27
1995-05-02
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435233, 435234, 43525231, 43525233, 43525235, 43525421, 4353201, 536 232, 536 237, C12N 119, C12N 121, C12N 1561, C12N 1563
Patent
active
054118867
ABSTRACT:
A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus characterized in that the xylose isomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95.degree. C. and the molecular weight of about 44,000, and is stabilized with manganese or magnesium. A process for preparing a xylose isomerase comprising transforming a microorganism with a plasmid containing the above gene and a promoter, culturing the transformed microorganism and harvesting the produced xylose isomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xylose isomerase.
REFERENCES:
patent: 4352881 (1982-10-01), Inagawa et al.
patent: 4593001 (1986-03-01), Horwarth
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5041378 (1991-08-01), Drummond et al.
patent: 5310665 (1994-05-01), Lambeir et al.
patent: 5340738 (1994-08-01), Lambeir et al.
Lehmacher et al., "Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8", 1990, J. Gen. Microb. 136: 679-686.
Scallet et al, "Studies in the Isomerization of D-Glucose", Starch, Dec. 1974, vol. 26, No. 12, pp. 405-408.
Lee et al, "Cloning and Expression of the Clostridium thermosulfurogenes Glucose Isomerase Gene in Escherichia coli and Bacillus subtilis", Applied and Environmental Microbiology, vol. 56, No. 9, Sep. 1990, pp. 2638-2643.
Dekker et al, "Xylose (Glucose) Isomerase Gene from the Thermophile Thermus thermophilus: Cloning, Sequencing, and Comparison with Other Thermostable Xylose Isomerases", Journal of Bacteriology, May 1991, vol. 173, No. 10, pp. 3078-3083.
Lehmacher et al., Biol. Chem. Hoppe-Seyler 371:527-536, Jun. 1990.
Stevis et al., Enzyme Microb. Technol. 7:592-596, Dec. 1985.
Adachi et al., J. Bacteriology 171:1010-1016, Feb. 1989.
Seno et al., Mol. Gen. Genet. 193:119-125, 1984.
Dekker Koen
Sakaguchi Kenji
Udaka Shigezo
Yamagata Hideo
Nihon Shokuhin Kako Co. Ltd.
Udaka Shiogezo
Walsh Stephen G.
LandOfFree
Xylose isomerase gene of Thermus aquaticus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Xylose isomerase gene of Thermus aquaticus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Xylose isomerase gene of Thermus aquaticus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1136258