Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2005-07-15
2011-10-11
Fronda, Christian (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S252300, C435S254110, C435S254200, C536S023200
Reexamination Certificate
active
08034591
ABSTRACT:
The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerizing xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of unspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.
REFERENCES:
patent: 02/38740 (2002-05-01), None
patent: 03/062430 (2003-07-01), None
patent: WO 03/062430 (2003-07-01), None
International Search Report PCT/NL2005/000516.
Marko Kuyper et al., Minimal metabolic engineering ofSaccharomyces cerevisiaefor efficient anaerobic xylose fermentation: a proof of principle (Fems Yeast Research, XP-002312911), Mar. 2004, pp. 655-664, vol. 4, No. 6, published by Elsevier.
K. L. Traff et al., Deletion of the GRE3 Aldose Reductase Gene and its influence on Xylose Metabolism in Recombinant Strains ofSaccharomyces cereviaseExpressing the xyIA and XKS1 Genes, (Applied and Environmental Microbiology-XP-002312912), Dec. 2001, pp. 5668-5674, vol. 67, No. 12, published by American Society for Microbiology.
Marko Kuyper et al., High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose bySaccharomyces cerevisiae?, (Fems Yeast Research, XP002312913), Oct. 2003, pp. 69-78, vol. 4, No. 1, published by Elsevier.
Lonn A. et al., Xylose isomerase activity influences xylose fermentation with recombinantSaccharomyces cerevisiaestrains expressing mutated xylA fromThermus thermophilus, (Enzyme and Microbial Technology, XP 002312914), Apr. 2003, pp. 567-573, vol. 32, No. 5, published by Elsevier.
Bjorn Johansson et al., The non-oxidative pentose phosphate pathway controls the fermentation rate xylose but not of xylose inSccharomyces cerevisiaeTMB3001, (Fems Yeast Research, XP-002966585), 2002, vol. 2, pp. 277-282, published by Elsevier Science, Tokyo, NL.
Kaisa Karhumaa et al., Investigation of limiting metabolic steps in the utilization of xylose by recombinantSaccharomyces cerevisiaeusing metabolic engineering, Yeast, 2005, vol. 22, pp. 359-368, published online Wiley InterScience.
De Laat Wilhelmus Theodorus Antonius Maria
Kuyper Sipko Maarten
Pronk Jacobus Thomas
Van Dijken Johannes Pieter
Winkler Aaron Adriaan
Browdy and Neimark PLLC
Fronda Christian
Techische Universiteit Delft
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