Xylose-fermenting recombinant yeast strains

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S014000, C435S041000, C435S254100, C435S255100, C435S007310, C435S006120, C435S254210

Reexamination Certificate

active

11398807

ABSTRACT:
Disclosed are xylose-fermenting recombinant yeast strains expressing xylose reductase, xylitol dehydrogenase, and xylulokinase and having reduced expression of PHO13 or a PHO13 ortholog, as well as methods of fermenting xylose to obtain ethanol using the recombinant yeast strains.

REFERENCES:
patent: 5789210 (1998-08-01), Ho et al.
patent: 6071729 (2000-06-01), Jeffries et al.
patent: 2003/0157675 (2003-08-01), Otero
patent: 2004/0142456 (2004-07-01), Jeffries et al.
Kaneko et al. ( Mol Gen Genet, 1989, 220, 133-139) abstract only.
Tuleva et al. ( FEMS Microbio. Lett. 1998, 161, 139-144).
Barua, M. et al., “Partial purification and characterization of a phosphoprotein phosphatase from sperm plasma membrane,” Reprod. Fertil. Dev. (1999) 11:379-386.
Bieche, I. et al., “Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer,” Int. J. Cancer (1998) 78:661-666.
Boeke, J.D. et al., “Ty elements transpose through an RNA intermediate,” Cell (1985) 40:491-500.
Chang, S.F. and Ho, N.W., “Cloning the yeast xylulokinase gene for the improvement of xylose fermentation,” Scientific Note. App. Biochem. Biotechnol. (1988) 17:313-318.
Chiang, L.-C. et al., “D-xylulose fermentation to ethanol byS. cerevisiae,” Appl. Environ. Microbiol. (1981) 42:284-289.
Cho, J-Y et al., “Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions,” App. Environ. Microbiol. (1998) 64(4):1350-1358.
Cho, K.M. et al., “Delta-integration of endo/exo-glucanase and beta-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol,” Enzyme Microb. Technol. (1999) 25:23-30.
Christianson, T.W. et al., “Multifunctional yeast high-copy-number shuttle vectors,” Gene (1992) 110:119-122.
Christova, N. and Galabova, D., “Phosphorylase phosphatase activity inSaccharomyces cerevisiae,” 257.Z Naturforsch (1998) [C] 53:951-956.
Crabtree, H.G., “Observations of the carbohydrate metabolism in tumors,” Biochem. J. (1929) 23:536-545.
De Preter, K. et al., “Quantification of MYCN, DDX1 and NAG gene copy number in neuroblastoma using a real-time quantitative PCR assay,” Mod. Pathol. (2002) 15:159-166.
Deng, X.X. and Ho, N.W., “Xylulokinase activity in various yeasts includingSaccharomyces cerevisiaecontaining the cloned xylulokinase gene,” Scientific Note. Appl. Biochem. Biotechnol. (1990) 24-25:193-199.
Eisen, M.B., et al., “Cluster analysis and display of genome-wide expression patterns,” Proc. Natl. Acad. Sci. USA (1998) 94:14863-14868.
Eliasson, A. et al., “Anaerobic xylose fermentation by recombinantSaccharomyces cerevisiaecarrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures,” App. Environ. Microbiol. (2000) 66:3381-3386.
Epstein, C.B. et al., “Genome-wide responses to mitochondrial dysfunction,” Mol. Biol. Cell (2001) 12:297-308.
Forsburg, S.L. and Guarente, L., “Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer,” Genes Dev. (1989) 3:1166-1178.
Hereford, J.B. and Rosbash, M., “Number and distribution of poly-adenylated RNA sequences in yeast,” Cell (1977) 10:453-462.
Herrero, P. et al., “Transcriptional regulation of theSaccharomyces cerevisiaeHXK1, HXK2 and GLK1 genes,” Yeast (1995) 11:137-144.
Hinnebusch, A.G., “Translational regulation of yeast GCN4. A window on factors that control initiator-trna binding to the ribosome,” J. Biol. Chem. (1997) 272:21661-21664.
Ho, N.W.Y. et al., “Genetically engineeredSaccharomycesyeast capable of effective cofermentation of glucose and xylose,” Appl. Environ. Microbiol. (1998) 64(5):1852-1859.
Hodges, P.E., “The yeast protein database (YPD): a curated proteome database forSaccharomyces cerevisiae,” Nucleic Acids Res. (1998) 26:68-72.
Hohmann, S. et al., “Evidence for trehalose-6-phosphate-dependent and -independent mechanisms in the control of sugar influx into yeast glycolysis,” Mol. Microbiol. (1996) 20:981-991.
Holstege, F.C. et al., “Dissecting the regulatory circuitry of a eukaryotic genome,” Cell (1998) 95:717-728.
Horiuchi, H. et al., “High level secretion of aRhizopus niveusaspartic proteinase inSaccharomyces cerevisiae,” Agric. Biol. Chem. (1990) 54(7):1771-1779.
Huh, W-K et al., “Molecular cloning and functional expression of alternative oxidase fromCandida albicans,” J. Bact. (1999) 181(13):4098-4102.
Ingham, D.J. et al., “Quantitative real-time PCR assay for determining transgene copy number in transformed plants,” Biotechniques (2001) 31:132-134, 136-140.
Jin, Y-S. et al., “Genome-wide expression analysis of xylose metabolism in recombinantSaccharomyces cerevisiaeexpressing PsXYL1, PsXYL2, and PsXYL3,” (Apr. 28, 2002) XP002376074 [Retrieved from the Internet http://www.ct.ornl.gov/symposium/24th/index—files/O2—04.htm, on Mar. 20, 2006].
Jin, Y.S. and Jeffries, T.W., “Changing flux of xylose metabolites by altering expression of xylose reductase and xylitol dehydrogenase in recombinantSaccharomyces cerevisiae,” Appl. Biochem. Biotechnol. (2002) 105-108:277-286.
Jin, Y.S. et al., “Molecular cloning of XYL3 (D-xylulokinase) fromPichia stipitisand characterization of its physiological function,” Appl. Environ. Microbiol (2002) 68:1232-1239.
Jin, Y.S. et al., “Conversion of xylose to ethanol by recombinantSaccharomyces cerevisiaecontaining genes for xylose reductase and xylitol dehydrogenase fromPichia stipitis,” J. Microbiol. Biotechnol. (2000) 10:564-567.
Jin, Y.S. et al., “Optimal growth and ethanol production from xylose by recombinantSaccharomyces cerevisiaerequire moderate D-xylulokinase activity,” Appl. Environ. Microbiol. (2003) 69:495-503.
Johansson, B. et al., “Xylulokinase overexpression in two strains ofSaccharomyces cerevisiaealso expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate,” App. Environ. Microbiol. (2001) 67:4249-4255.
Kaneko, Y. et al., “Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation inSaccharomyces cerevisiae,” Mol. Gene. Genet. (1989) 220:133-139.
Kingsman, A.J. and Kingsman, S.M., “Ty: A retroelement moving forward,” Cell (1988) 53:333-335.
Kotter, P. and Ciriacy, M., “Xylose fermentation bySaccharomyces cerevisiae,” Appl. Microbiol. Biotechnol. (1993) 38:776-783.
Kurtzman, C.P., “Molecular taxonomy of the yeasts,” Yeast (1994) 10:1727-1740.
Lai, K. and Elsas, L.J., “Overexpression of human UDP-glucose pyrophosphorylase rescues galactose-1-phosphate uridyltransferase-deficient yeast,” Biochem. Biophys. Res. Commun. (2000) 271:392-400.
Maleszka, R. and Schneider, H., “Involvement of oxygen and mitochondrial function in the metabolism of D-xylulose bySaccharomyces cerevisiae,” Arch. Biochem. Biophys. (1984) 228:22-30.
Ndubuisil, M.I. et al., “Characterization of a novel mammalian phosphatase having sequence similarity toSchizosaccharomyces pombePHO2 andSaccharomyces cerevisiaePHO13,” Biochem. (2002) 41:7841-7848.
Nissen, T.L. et al., “Anaerobic and aerobic batch cultivations ofSaccharomyces cerevisiaemutants impaired in glycerol synthesis,” Yeast (2000) 16:463-474.
Olesen, J. et al., “Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner,” Cell (1987) 51:953-961.
Parekh, R.N. et al., “An integrating vector for tunable, high copy, stable integration into the dispersed Ty delta sites ofSaccharomyces cerevisiae,” Biotechnol. Prog. (1996) 12:16-

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