Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-11-13
1999-09-07
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435325, 4352523, 43525411, 536 232, 536 243, C12N 924, C12N 1556
Patent
active
059486678
ABSTRACT:
A xylanase gene, denoted xynC, encoding a novel xylanase (XynC) obtained from the anaerobic fungus Neocallimastix patriciarum is provided. The DNA sequence of the xynC gene is also provided. Transformation of microbial and plant hosts with the xynC gene is described. The xynC gene may be used to design probes for use in hybridization experiments to isolate xylanase genes from other anaerobic fungi. The xynC gene has been used to construct an oleosin-xynC expression construct encoding an oleosin-xylanase fusion protein which retains xylanase activity. Transgenic Brassica napus (canola), transformed with the oleosin-xynC expression construct, expresses the oleosin-xylanase fusion protein which is immobilized in the oil-body membrane of the B. napus seeds. Canola meal, the protein-rich residue left after canola oil is extracted from canola plants, when derived from the transgenic B. napus of the present invention, retains substantial xylanase activity, making it an ideal animal feed supplement.
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Cheng Kuo-Joan
Forsberg Cecil Wallace
Hu Youji
Liu Jin-Hao
Moloney Maurice M.
Her Majesty the Queen in right of Canada as represented by the
Patterson Jr. Charles L.
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