Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Patent
1995-02-03
1997-11-11
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
435 6, 435 721, 435 724, 435 731, C12Q 102, C12Q 168
Patent
active
056862610
ABSTRACT:
The present invention describes a method of staining mitochondria, and analyzing mitochondrial function, using a class of fluorescent substituted 3',6'-diaminoxanthenes and their reduced analogs 3',6'-diaminodihydroxanthenes, which are oxidized to the fluorescent form of the dye in situ. In their oxidized form, the dyes selectively localize within mitochondria. The dyes of the invention are substituted by an alkylating group that allows their retention in mitochondria even after cell death, fixation, and permeabilization.
REFERENCES:
patent: 5321130 (1994-06-01), Yue et al.
Richard P. Haugland, Molecular Probes, Handbood of Fluorescent Probes and Research Chemicals 1992-1994, Molecular Probes, Inc. (1992). pp. 9-16 1992.
Michael Brinkley, Bioconjugate Chemistry, vol. 3, pp. 2-13 (1992).
Haugland, Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, 1992, Set. 30.
Rothe, et al., Journal of Immunological Methods, 138, 133 (1991).
Martinez, et al., Exp. Cell Res., 164, 551 (1986).
Kliot-Fields, et al., Somatic Cell Genetics, 9, 375 (1983).
Riedy, et al., Cytometry, 12, 133 (1991).
Haugland, Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, 1992, Set 24.
Hahn, et al., J. Histochem. Cytochem., 41, 631 (1993).
Haugland Richard P.
Zhang Yu-Zhong
Ceperley Mary E.
Helfenstein Allegra J.
Molecular Probes, Inc.
Skaugset Anton E.
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