Xanthan-ester and acridan substrates for horseradish peroxidase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 435 28, 435188, 435968, 546102, 546104, 549 24, 549223, 549225, G01N 33533, C07D21904, C07D31182, C07D33512, C12N 996

Patent

active

061626104

ABSTRACT:
Xanthan esters and acridans are substrates for horseradish peroxidase. These stable, enzymatically cleavable chemiluminescent esters are substrates for horseradish peroxidase which, together with peroxide is among the extensively used enzyme in enzyme-linked detection methods, including immunoassays, oligonucleotide detection and nucleic acid hybridization. The novel compounds are used, together with peroxide, alkali and the peroxidase, to indicate the presence and/or concentration of target compounds. The assays may be enhanced by the use of polymeric quaternary onium enhancement compounds or similar compounds selected to enhance the chemiluminescence emitted.

REFERENCES:
patent: 5241070 (1993-08-01), Law et al.
patent: 5290936 (1994-03-01), Beheshti et al.
patent: 5523212 (1996-06-01), Akhavan-Tafti et al.
patent: 5593845 (1997-01-01), Akhavan-Tafti et al.

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