β, β-carotene 15, 15′-monooxygenases,...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S183000, C435S252300, C435S320100, C435S325000, C435S410000, C435S006120, C536S023200, C536S022100, C536S024100

Reexamination Certificate

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06897051

ABSTRACT:
Nucleic acid sequences coding for a protein having β,β-carotene 15,15′-monooxygenase activity and their uses in diagnostics, the synthesis of vitamin A and methods for the introduction of the β,β-carotene 15,15′-monooxygenase cDNA into host cells are disclosed.

REFERENCES:
patent: 4727028 (1988-02-01), Santerre et al.
patent: WO 9323538 (1993-11-01), None
Takayama et al. Antisense RNA.Crit Rev Biochem Mol Biol. 1990;25(3):155-84.*
Wyss et al. Expression pattern and localization of beta,beta-carotene 15,15′-dioxygenase in different tissues. Biochem J. Mar. 15, 2001;354(Pt 3):521-9.*
Wyss et al. Cloning and expression of beta,beta-carontene 15,15′-dioxygenase. Biochem. Biophys Res Commun. Mar 10, 2000;271(2):334-6.*
Leuenberger et al. The Reaction Mechanism of the Enzyme-Catalyzed Central Cleavage of beta-Carotene to Retinal. Angew Chem Int Ed Engl. Jul. 16, 2001;40(14):2613-2617.*
Lakshmanan, et al., “Purification and Properties of Carotene 15, 15′-Dioxygenase of Rabbit Intestine,”Journal of Lipid Research, vol. 13, pp. 477-482 (1972).
Devery, et al., “β-Carotene-15, 15′-Dioxygenase (EC 1.13.11.21) Isolation Reaction Mechanism and an Improved Assay Procedure,”British Journal of Nutrition, vol. 72, pp. 397-414 (1994).
Wolf, “The Enzymatic Cleavage of β-Carotene: Still Controversial,”Nutrition Reviews, vol. 53, No. 5, pp. 134-137 (1995).
Thorbjornson, et al., GenEmbl database—Accession No. AF073473 (1998).

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