β-secretase enzyme compositions and methods

Details β-secretase enzyme compositions and methods β-secretase enzyme compositions and methods

2006-10-03

2006-10-03

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C530S300000, C435S320100, C435S325000, C536S023200

Reexamination Certificate

active

07115410

ABSTRACT:
Disclosed are various forms of an active, isolated β-secretase enzyme in purified and recombinant form. This enzyme is implicated in the production of amyloid plaque components which accumulate in the brains of individuals afflicted with Alzheimer's disease Recombinant cells that produce this enzyme either alone or in combination with some of its natural substrates (β-APPwt and β-APPsw) are also disclosed, as are antibodies directed to such proteins. These compositions are useful for use in methods of selecting compounds that modulate β-secretase. Inhibitors of β-secretase are implicated as therapeutics in the treatment of neurodegenerative diseases, such as Alzheimer's disease.

REFERENCES:
patent: 5424205 (1995-06-01), Dovey et al.
patent: 5455169 (1995-10-01), Mullan
patent: 5593846 (1997-01-01), Schenk et al.
patent: 5733768 (1998-03-01), Dixon et al.
patent: 5744346 (1998-04-01), Chrysler et al.
patent: 5750349 (1998-05-01), Suzuki et al.
patent: 5766846 (1998-06-01), Schlossmacher et al.
patent: 5795963 (1998-08-01), Mullan
patent: 5837672 (1998-11-01), Schenk et al.
patent: 5849560 (1998-12-01), Abraham
patent: 5863756 (1999-01-01), Barr et al.
patent: 5877015 (1999-03-01), Hardy et al.
patent: 5942400 (1999-08-01), Anderson et al.
patent: 6025180 (2000-02-01), Powell et al.
patent: 6162630 (2000-12-01), Powell et al.
patent: 6211428 (2001-04-01), Singh et al.
patent: 6221645 (2001-04-01), Chrysler et al.
patent: 6245884 (2001-06-01), Hook
patent: 6245964 (2001-06-01), McConlogue et al.
patent: 6313268 (2001-11-01), Hook
patent: 6319689 (2001-11-01), Powell et al.
patent: 6329163 (2001-12-01), Anderson et al.
patent: 6358725 (2002-03-01), Christie et al.
patent: 6361975 (2002-03-01), Christie et al.
patent: 6420534 (2002-07-01), Gurney et al.
patent: 6440698 (2002-08-01), Gurney et al.
patent: 6500667 (2002-12-01), Gurney et al.
patent: 6627739 (2003-09-01), Anderson
patent: 6699671 (2004-03-01), Gurney et al.
patent: 6706485 (2004-03-01), Gurney et al.
patent: 6727074 (2004-04-01), Gurney et al.
patent: 6737510 (2004-05-01), Gurney et al.
patent: 6753163 (2004-06-01), Gurney et al.
patent: 6790610 (2004-09-01), Gurney et al.
patent: 6797487 (2004-09-01), Gurney et al.
patent: 6825023 (2004-11-01), Gurney et al.
patent: 6828117 (2004-12-01), Gurney et al.
patent: 6835565 (2004-12-01), Gurney et al.
patent: 6844148 (2005-01-01), Gurney et al.
patent: 6852482 (2005-02-01), Chrysler et al.
patent: 2001/0016324 (2001-08-01), Gurney et al.
patent: 2001/0018208 (2001-08-01), Gurney et al.
patent: 2001/0021391 (2001-09-01), Gurney et al.
patent: 2002/0037315 (2002-03-01), Gurney et al.
patent: 2002/0064819 (2002-05-01), Gurney et al.
patent: 2002/0081634 (2002-06-01), Gurney et al.
patent: 2003/0077226 (2003-04-01), Gurney et al.
patent: 2003/0104365 (2003-06-01), Gurney et al.
patent: 2004/0043408 (2004-03-01), Gurney et al.
patent: 2004/0048303 (2004-03-01), Gurney et al.
patent: 2004/0166507 (2004-08-01), Gurney et al.
patent: 2004/0234976 (2004-11-01), Gurney et al.
patent: 0 848 062 (1998-06-01), None
patent: 0 855 444 (1998-07-01), None
patent: 855 442 (1998-07-01), None
patent: 1 445 263 (2004-08-01), None
patent: 2 364 059 (2004-01-01), None
patent: 2 389 113 (2004-02-01), None
patent: 2 389 114 (2004-02-01), None
patent: 2 389 182 (2004-02-01), None
patent: WO 96/20725 (1996-07-01), None
patent: WO 96/31122 (1996-10-01), None
patent: WO 96/40885 (1996-12-01), None
patent: WO 97/47314 (1997-12-01), None
patent: WO 98/11236 (1998-03-01), None
patent: WO 98/13488 (1998-04-01), None
patent: WO 98/21589 (1998-05-01), None
patent: WO 98/22597 (1998-05-01), None
patent: WO 98/26059 (1998-06-01), None
patent: WO 98/37226 (1998-08-01), None
patent: WO 99/31236 (1999-06-01), None
patent: WO 99/34004 (1999-08-01), None
patent: WO 99/46281 (1999-09-01), None
patent: WO 99/64587 (1999-12-01), None
patent: WO 00/17369 (2000-03-01), None
patent: WO 00/23576 (2000-04-01), None
patent: WO 00/47618 (2000-08-01), None
patent: WO 00/56871 (2000-09-01), None
patent: WO 00/58479 (2000-10-01), None
patent: WO 00/58479 (2000-10-01), None
patent: WO 00/68266 (2000-11-01), None
patent: WO 00/69262 (2000-11-01), None
patent: WO 01/00663 (2001-01-01), None
patent: WO 01/00665 (2001-01-01), None
patent: WO 01/23533 (2001-04-01), None
patent: WO 01/29563 (2001-04-01), None
patent: WO 01/31054 (2001-05-01), None
patent: WO 01/36600 (2001-05-01), None
patent: WO 01/38487 (2001-05-01), None
patent: WO 01/49097 (2001-07-01), None
patent: WO 01/49098 (2001-07-01), None
patent: WO 01/50829 (2001-07-01), None
Sinha S. et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain, Nature, 1999, 402, 537-504.
Yan R. et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity, Nature, 1999, 402, 533-537.
Vassar R. et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by transmembrane aspartic protease BACE, Science, 199, 286, 735-741.
Tang J. et al. Evolution in Structure and Function of Aspartyl Proteases, Journal of Cellular Biochemistry, 1987, 33, 53-63.
Viswandhan V. et al. An Approach to Rapid Estimation of Relative Binding Affinities of Enzyme Inhibitors: Application to Peptidomimetics Inhibitors of the Human immunodeficiency Virus Type 1 protease, J. Med. Chem. 1996, 39, 705-712.
Kuntz I. Structure-Based Strategy for Drug Design and Discovery, Science, 1992, 257, 1078-1082.
Beyer B. M. et al. Self-activation of Recombinant Human Lysosomal Procathepsin D at a Newely Engineered Cleavage, Junction, J. Biol. Chem. 1996, vol. 271, 15590-15596.
Azuma T. et al., Human Gastric Cathepsin E, J. Biol. Chem. 1989, 264, 16748-16753.
Athauda S. et al, Autocatalytic Processing of Procathepsin E to Cathepsin E and Their Structural Differences, Biochem. Biophys. Res. Commun. 1991, 175, 152-158.
U.S. Appl. No. 60/141,363, Lin et al.
U.S. Appl. No. 60/139,172, Anderson et al.
U.S. Appl. No. 60/119,571, Basi et al.
U.S. Appl. No. 60/114,408, Basi et al.
U.S. Appl. No. 60/101,594, Gurney et al.
Baldwin, et al., Crystal structures of native and inhibited forms of human cathepsin D: Implications for lysosomal targeting and drug design,PNAS USA, 90:6796-6800 (1993).
Brown, et al., Evaluation of Cathepsins D and G and EC 3.4.24.15 as Candidate β-Secretase Proteases Using Peptide and Amyloid Precursor Protein Substrates,Journal of Neurochemistry, 66: 2436-2445 (1996).
Chevallier, et al., Cathepsin D displays in vitro β-secretase-like specificity,Brain Research, 750:11-19 (1997).
Chyung, et al. Novel β-Secretase Cleavage of β-Amyloid Precursor Protein in the Endoplasmic Reticulum/Intermediate Compartment of NT2N Cells,Journal of Cell Biology, 138: 671-680 (Aug. 11, 1997).
Diedrich, et al., Nucleotide sequence of a cDNA encoding mouse cathepsin D,Nucleic Acids Research, 18:7184 (1990).
Elan Corporation, plc and Pharmacia Corporation announce research collaboration, News Aug. 9, 2000, www.elancorp.com.
Evin, et al., Alzheimer's disease amyloid precursor protein (βAPP): proteolytic processing, secretases and βA4 amyloid production,Amyloid; Int. J. Exp. Clin. Invest.1: 263-280 (Sep. 8, 1994).
Haass, et al., Amyloid β-peptide is Produced by Cultured Cells During Normal Metabolism,Nature, 359: 322-325 (Sep. 24, 1992).
Haass, et al., The Swedish Mutation Causes Early-Onset Alzheimer's Disease by β-Secretase Cleavage Within the Secretory Pathway,Nature Medicine, 12: 1291-1296 (Dec. 1995).
Haass, et al., β-Amyloid Peptide and 3-kDa Fragment are Derived by Distinct Cellular Mechanisms,Journal of Biochemistry, 268: 3021-3024 (Feb. 15, 1993).
Hirosawa, et al., Characterization of cDNA Clones Selected by the GeneMark Analysis from Size-Fractionated cDNA Libraries From Human Brain,DNA Res., 6(5): 329-336 (Oct. 29, 1999).
Hussain, et al., Identification of a Novel Aspartic Protease (Asp 2) as β-Secretase,Molecular and Cellular Neuroscience, 14: 419-427 (1999).
Kang, et al., The Precursor of Alzheimer's Disease Amyloid A4 Protein Resembles a Cell-Surface Receptor,Nature, 325

Affiliated with

Also associated with

Rating

β-secretase enzyme compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with β-secretase enzyme compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and β-secretase enzyme compositions and methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3631682