β-galactoside-α2,6-sialyltransferase, a gene...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S006120, C435S007100, C530S350000, C530S300000

Reexamination Certificate

active

07993875

ABSTRACT:
The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.

REFERENCES:
patent: 6255094 (2001-07-01), Yamamoto et al.
patent: 0915163 (1999-05-01), None
patent: WO-9838315 (1998-09-01), None
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Yamamoto, T. et al., “Cloning and Expression of a Marine Bacterial β-Galactoside α2,6-Sialyltransferase Gene fromPhotobacterium damselaJT0160”, Journal of Biochemistry, vol. 123, No. 1, 1998, pp. 94-100.
Hamamoto, T. et al., “Two Step Single Primer Mediated Polymerase Chain Reaction.1 Application to Cloning of Putative Mouse, β-Galactoside α2,6-Sialyltransferase cDNA”, Bioorganic & Medicinal Chemistry vol. 1, No. 2, pp. 141-145, 1993.
Yu, H. et al., “A Multifunctional Pasteurella multocida Sialytransferase: A Powerful Tool for the Synthesis of Sialoside Libraries”, Journal of the American Chemical Society, vol. 127, No. 50, 2005, pp. 17618-17619.
Yamamoto, T. et al., “Mass Production of Bacterial α2,6-Sialytransferase and Enzymatic Syntheses of Sialyloligosaccharides”, Biosci. Biotechnol. Biochem., vol. 62, No. 2, pp. 210-214, 1998, XP001069298.
Yamamoto, T. et al., “Purification and Characterization of a Marine Bacterial β-Galactoside α2,6-Sialyltransferase fromPhotobacterium damselaJT0160”, Journal of Biochemistry, vol. 120, No. 1, 1996, pp. 104-110, XP001069919.

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