α-amylase variants

Details α-amylase variants α-amylase variants

2005-01-19

2009-10-13

Hutson, Richard G (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S204000, C435S210000, C510S226000, C510S236000, C510S530000, C510S320000, C510S392000

Reexamination Certificate

active

07601527

ABSTRACT:
The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.

REFERENCES:
patent: 5731280 (1998-03-01), Nielsen et al.
patent: WO 90/11352 (1990-10-01), None
patent: WO 95/10603 (1995-04-01), None
patent: WO 95/26397 (1995-10-01), None
patent: WO 96/23873 (1996-08-01), None
patent: WO 96/23874 (1996-08-01), None
patent: WO 97/41213 (1997-11-01), None
patent: WO 97/43424 (1997-11-01), None
patent: WO 98/26078 (1998-06-01), None
patent: WO 99/19467 (1999-04-01), None
Ngo et al., in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Conrad et al., Eur. J. Biochem. 230:481-490 (1995).
A. Kadziola, Ph.D., Thesis “An alpha-amylase from Barley and its Complex with a Substrate Analogue Inhibitor Studied by X-ray Crystallography”, Department of Chemistry, University of Copenhagen (1993).
Klein, C., et al.,Biochemistry 1992, 31, 8740-8746.
Mizuno, H., et al.,J. Mol. Biol. (1993) 234, 1282-1283.
Chang. C., et al,J. Mol. Biol. (1993) 229, 235-238.
Larson, S.B.,J. Mol. Biol. (1994) 235, 1560-1584.
Lawson, C.L.,J. Mol. Biol. (1994) 236, 590-600.
Qian, M., et al.,J. Mol. Biol. (1993) 231, 785-799.
Brady, R.L., et al.,Acta Crystallogr. sect. B, 47, 527-535, 1991.
Swift, H.J., et al.,Acta Crystallogr. sect. B, 47, 535-544, 1991.
MacGregor, E.A., Food Hydrocolloids, 1987, vol. 1, No. p. 5-6, B. Diderichsen and L. Christiansen, Cloning of a maltogenic α-amylase fromBacillus stearothermophilus, FEMS Microbiol. letters: 56: pp. 53-60 (1988).
Matthes et al.,The EMBO J. 3. 1984, pp. 801-805.
R.K. Saiki et al.,Science 239, 1988, pp. 487-491.
Morinaga et al., (1984, Biotechnology 2:646-639).
Nelson and Long,Analytical Biochemistry 180, 1989, pp. 147-151.
Hunkapiller et al., 1984, Nature 310:105-111.
R. Higuchi, B. Krummel, and R.K. Saiki (1988). A general method of in vitro preparation and specific muta-genesis of DNA fragments: study of protein and DNA interactions.Nucl. Acids Res. 16:7351-7367.
Dubnau et al., 1971,J. Mol. Biol. 56, pp. 209-221.
Gryczan et al., 1978,J. Bacteriol. 134, pp. 318-329.
S.D. Erlich, 1977,Proc. Natl. Acad. Sci. 74, pp. 1680-1682.
Boel et al., 1990,Biochemistry 29. pp. 6244-6249.
MacGregor, E.A., Food Hydrocolloids, 1987, vol. 1, No. p. 5-6, B.
Diderichsen and L. Christiansen, Cloning of a maltogenic α-amylase fromBacillus stearothennophilus, FEMS Microbiol. letters: 56: pp. 53-60(1988).
Beaucage et al., “Deoxynucleoside Phosphoramidites - A New Class . . . ”, Tetrahedron Letters, vol. 22, No. 20, pp. 1859-1862 (1981).

Affiliated with

Also associated with

Rating

α-amylase variants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with α-amylase variants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and α-amylase variants will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4134697