α-amylase variants

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Details

C536S023100, C536S023700, C435S202000, C435S204000, C435S210000, C510S226000, C510S236000, C510S530000, C510S320000, C510S392000

Reexamination Certificate

active

06887986

ABSTRACT:
The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.

REFERENCES:
patent: 5731280 (1998-03-01), Nielsen et al.
patent: WO 9011352 (1990-10-01), None
patent: WO 9510603 (1995-04-01), None
patent: WO 9526397 (1995-10-01), None
patent: WO 9623873 (1996-08-01), None
patent: WO 9623874 (1996-08-01), None
patent: WO 9741213 (1997-11-01), None
patent: WO 9743424 (1997-11-01), None
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.

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