α-amylase mutants

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S202000, C536S023200

Reexamination Certificate

active

11064196

ABSTRACT:
The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprisesi) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations),ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally,iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.

REFERENCES:
patent: 4600693 (1986-07-01), Kindle et al.
patent: 5830837 (1998-11-01), Bisg.ang.rd-Frantzen et al.
patent: 0 410 498 (1990-06-01), None
patent: WO 91/00343 (1991-01-01), None
patent: WO 94/18314 (1994-08-01), None
patent: WO 95/35382 (1995-12-01), None
Holm et al. (1990) Protein Engineering, vol. 3(3), pp. 181-191.
Kim et al (1994) Bull. Korean Chem. Soc., vol. 15 (10), pp. 832-835.
Chang et al., “Crystallization and Preliminary X-ray Crystallographic Analysis of α-Amylase FromBacillus subtilis”, J. Mol. Biol., 1993, 229, pp. 235-238.
Gray et al., “Structural Genes Encoding The Thermophilic α-Amylases ofBacillus stearothermophilusAndBacillus licheniformis”, Journal of Bacteriology, vol. 166, No. 2, May 1996, pp. 635-643.

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