α-2,3-Sialyltransferase polypeptides

Details α-2,3-Sialyltransferase polypeptides α-2,3-Sialyltransferase polypeptides

2006-04-11

2006-04-11

Swope, Sheridan (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S069700

Reexamination Certificate

active

07026147

ABSTRACT:
This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example,Campylobacterspecies, includingC. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

REFERENCES:
patent: 5352670 (1994-10-01), Venot et al.
patent: 5374541 (1994-12-01), Wong et al.
patent: 5545553 (1996-08-01), Gotschlich
patent: WO 92/16640 (1992-10-01), None
patent: WO 95/02683 (1995-12-01), None
patent: WO 96/32491 (1996-10-01), None
patent: WO 99/49051 (1999-09-01), None
patent: WO 00/46379 (2000-08-01), None
Guerry et al, Sialylation of lipooligosaccharide cores affects immunogenicity and serum resistance ofCampylobacter jejuni. Infect Immun. Dec 2000;68(12):6656-62.
Hood et al, Identification of a lipopolysaccharide alpha-2,3-sialyltransferase fromHaemophilus influenzae. Mol Microbiol. Jan 2001;39(2):341-50.
Eichler et al, Synthesis of a disialylated hexasaccharide of type VIII group BStreptococcuscapsular polysaccharide. Carbohydrate Res. Jun 1999;319:1-16.
Aspinall et al.; “Chemical Structures of the Core Regions ofCampylobacter JejuniSerotypes 0:1, 0:4, 0:23, and 0:36 Lipopolysaccharides”;European Journal of Biochemistry;May, 1993; pp. 1017-1027; vol. 213, No.3.
Aspinall et al.; “Lipopolysaccharides fromCampylobacter jejuniAssociated with Guillain-Barre Syndrome Patients Mimic Human Gangliosides in Structure”;Infect. Immun.;1994a; pp. 2122-2125; vol. 62.
Aspinall et al.; “Lipopolysaccharides ofCampylobacter jejuniSerotype O:19: Structures of Core Oligosaccharide Regions from the Serostrain and Two Bacterial Isolates from Patients with the Guillain-Barre Syndrome”;Biochemistry;1994b; pp. 241-249; vol. 33.
Aspinall et al.; “Lipopolysaccharides ofCampylobacter jejuniSerotype O:19: Antigen Chains from the Serostrain and Two Bacterial Isolates from Patient with the Guillain-Barre Syndrome”;Biochemistry;1994c; pp. 250-255; vol. 33.
Belunis et al.; “Biosynthesis of Endotoxin”;J. Biol. Chem.;1992; pp. 9988-9997; vol. 267.
Gaudino et al.; “A Novel and Efficient Synthesis of Neolacto Series Gangliosides 3′-nLM1and 6′-nLM1”;J. Am. Chem. Soc. ;1994; pp. 1149-1150; vol. 116.
Gilbert et al.; “Biosynthesis of Ganglioside Mimics inCampylobacter JejuniOH4384”;The Journal of Biolofical Chemistry;Feb. 11, 2000; pp. 2896-3906; vol. 275, No. 6; The American Society for Biochemistry and Molecular Biology, Inc.; USA.
Gilbert et al.; “Cloning of the Lipooligosaccharide α-2,3-Sialytransferase from the Bacterial PathogensNeisseria meningitidisandNeissera gonorrhoeae”; J. Biol. Chem.;1996; pp. 28271-28276; vol. 271.
Gilbert et al.; EMBL Acc# AY044868 2002. Alignment with Seq Id No: 3.
Ito et al.; “Synthesis of Bioactive Sialosides”;Pure Appl. Chem.;1993; p. 753; vol. 65.
Kuroki; “Campylobacter jejuniStrains from Patients with Guillain-Barre Syndrome Belong Mostly to Penner Serogroup 19 Contain β-N-Acetylglucosamine Residues”;Ann. Neurol.;1993; pp. 243-247; vol. 33.
Parkhill et al.; “The Genome Sequence of the Food-Borne PathogenCampylobacter JejuniReveals Hypervariable Sequences”;Nature;Feb. 10, 2000; pp. 665-668; vol. 403.
Penner et al.; “Diversity of Lipopolysaccharide Structures inCampylobacterJejuni”;The Journal of Infectious Diseases;Dec., 1997; pp. S135-S138; vol. 178, No. 2.
Prendergast et al.; “Lipopolysaccharides formCampylobacter JejuniO:41 Strains Associated with Guillain-Barré Syndrome Exhibit Mimicry of GM1 Ganglioside”;Infection and Immunity;Aug. 1998: pp. 3649-3677; vol. 66, No. 8.
Preston et al.; “The Lipooligosaccharides of Pathogenic Gram-Negative Bacteria”;Critical Reviews in Microbiology;1996; pp. 139-180; vol. 22. No. 3.
Sabesan et al.; “Combined Chemical and Enzymatic Synthesis of Sialyloligosaccharides and Characterization by 500-MHz1H and C NMR Spectroscopy”;J. Am. Chem. Soc.;1986; pp. 2068-2080; vol. 108.
Salloway et al.; “Miler-Fisher Syndrome Associated withCampylobacter jejuniBearing Lipopolysaccharide Molecules that Mimic Human Ganglioside GD”;Infect Immun.;1996; pp. 2945-2949; vol. 64.
Wakarchuk et al.; “Functional Relationships of the Genetic Locus Encoding the Glycosyltransferase Enzymes Involved in Expression of the Lacto-N-neotetraose Terminal Lipopolysaccharide Structure inNeisseria meningitidis”; J. Biol. Chem.;1996; pp. 19166-19173; vol. 271.
Wood et al.; “Cloning, Mutation and Distribution of a Putative Lipopolysaccharide Biosynthesis Locus in Campylobacter Jejuni”;Microbiology;Feb. 1999; pp. 379-388; vol. 145, No. 2.
Genbank Database Accession No. AL111168 Jul. 18, 2002, Parkhill et al.
http://web.archive.org/web/*/http://www.sanger.ac.uk/Projects/C—Jejuni/ Search Results for Jan. 1, 1996-Apr. 7, 2004.

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