Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2004-04-06
2009-12-08
McElwain, Elizabeth F (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S281000, C435S320100, C435S252300, C536S023200
Reexamination Certificate
active
07629503
ABSTRACT:
The present invention relates to an improved process for the specific preparation of unsaturated ω-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of ω-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having Δ-4 double bonds owing to the expression of a Δ-4-desaturase fromEuglena gracilis.The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof.Fatty acids and triglycerides have a large number of uses in the food industry, in animal nutrition, cosmetics and in the drugs sector. They are suitable for a wide variety of uses depending on whether they are free saturated or unsaturated fatty acids or triglycerides having an increased content of saturated or unsaturated fatty acids.
REFERENCES:
patent: 5614393 (1997-03-01), Thomas et al.
patent: 0 550 162 (1993-07-01), None
patent: 0 794 250 (1997-09-01), None
patent: WO-91/13972 (1991-09-01), None
patent: WO-93/06712 (1993-04-01), None
patent: WO-93/11245 (1993-06-01), None
patent: WO-94/11516 (1994-05-01), None
patent: WO-94/18337 (1994-08-01), None
patent: WO-95/18222 (1995-07-01), None
patent: WO-96/21022 (1996-07-01), None
patent: WO-97/21340 (1997-06-01), None
patent: WO-97/30582 (1997-08-01), None
patent: WO-98/46763 (1998-10-01), None
patent: WO-98/46764 (1998-10-01), None
patent: WO-98/46765 (1998-10-01), None
patent: WO-98/46776 (1998-10-01), None
patent: WO-99/27111 (1999-06-01), None
patent: WO-99/64616 (1999-12-01), None
patent: WO-00/21557 (2000-04-01), None
patent: WO-02/26946 (2002-04-01), None
patent: WO-02/090493 (2002-11-01), None
Barsanti, L. et al., “Fatty Acid Content in Wild Type and WZSL Mutant ofEuglena gracilis”, Journal of Applied Phycology, 2000, vol. 12, pp. 515-520.
Horrocks, L. A. et al., “Health Benefits of Docosahexaenoic Acid (DHA)”, Pharmacological Research, 1999, vol. 40, No. 3, pp. 211-225.
Huang, Y. S. et al., “Cloning of Δ12- and Δ6-Desaturases fromMortierella alpinaand Recombinant Production of γ-Linolenic Acid inSaccharomyces cerevisiae”, Lipids, 1999, vol. 34, No. 7, pp. 649-659.
Hunter, J. E., “Studies on Effects of Dietary Fatty Acids as Related to Their Position on Triglycerides”, Lipids, 2001, vol. 36, No. 7, pp. 655-668.
Alonso, D. L. et al., “Plants as ‘chemical factories’ for the Production of Polyunsaturated Fatty Acids”, Biotechnology Advances, 2000, vol. 18, pp. 481-497.
McKeon, T. et al., “Stearoyl-Acyl Carrier Protein Desaturase from Safflower Seeds”, Methods in Enzymology, 1981, vol. 71, pp. 275-281.
Meyer, A. et al., “Biosynthesis of Docosahexaenoic Acid inEuglena gracilis: Biochemical and Molecular Evidence for the Involvement of a Δ4-Fatty Acyl Group Desaturase”, Biochemistry, 2003, vol. 42, pp. 9779-9788.
Pereira, S. L. et al., “Recent Advances in the Study of Fatty Acid Desaturases from Animals and Lower Eukaryotes”, Prostaglandins, Leukotrienes and Essential Fatty Acids, 2003, vol. 68, pp. 97-106.
Poulos, A. “Very Long Chain Fatty Acids in Higher Animals—A Review”, Lipids, 1995, vol. 30, No. 1, pp. 1-14.
Sakuradani, E. et al., “Δ6-Fatty Acid Desaturase from an Arachidonic Acid-ProducingMortierellaFungus. Gene Cloning and its Heterologous Expression in a Fungus,Aspergillus”, Gene, 1999, vol. 238, pp. 445-453.
Stukey, J. E. et al., “TheOLE1Gene ofSaccharomyces cerevisiaeEncodes the Δ9 Fatty Acid Desaturase and Can Be Functionally Replaced by the Rat Stearoyl-CoA Desaturase Gene”, The Journal of Biological Chemistry, 1990, vol. 265, No. 33, pp. 20144-20149.
Voss, A. et al., “The Metabolism of 7, 10, 13, 16, 19-Docosapentaenoic Acid to 4, 7, 10, 13, 16, 19-Docosahexaenoic Acid in Rat Liver is Independent of a 4-Desaturase”, The Journal of Biological Chemistry, 1991, vol. 266, No. 30, pp. 19995-20000.
Wada, H. et al., “Enhancement of Chilling Tolerance of a Cyanobacterium by Genetic Manipulation of Fatty Acid Desaturation”, Nature, 1990, vol. 347, pp. 200-203.
Wallis, J. G. et al., “The Δ8-Desaturase ofEuglena gracilis: An Alternate Pathway for Synthesis of 20-Carbon Polyunsaturated Fatty Acids”, Archives of Biochemistry and Biophysics, 1999, vol. 365, No. 2, pp. 307-316.
Wang, X. et al., “Biosynthesis and Regulation of Linolenic Acid in Higher Plants”, Plant Physiol. Biochem., 1988, vol. 26, No. 6, pp. 777-792.
Yu, R. et al., “Production of Eicosapentaenoic Acid by a Recombinant Marine Cyanobacterium,Synechococcussp.”, Lipids, 2000, vol. 35, No. 10, pp. 1061-1064.
Zank, T. K. et al., “Cloning and Functional Characterisation of an Enzyme Involved in the Elongation of Δ6-Polyunsaturated Fatty Acids from the MossPhyscomitrella patens”, The Plant Journal, 2002, vol. 31, No. 3, pp. 255-268.
Bauer Jörg
Cirpus Petra
Heinz Ernst
Meyer Astrid
Zähringer Ulrich
BASF Plant Science GmbH
Connolly Bove & Lodge & Hutz LLP
McElwain Elizabeth F
LandOfFree
Δ-4 desaturases from Euglena gracilis , expressing... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Δ-4 desaturases from Euglena gracilis , expressing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Δ-4 desaturases from Euglena gracilis , expressing... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4058025