Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-05-08
2007-05-08
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S041000, C435S091100, C435S254100, C435S254200, C435S254110, C435S254220, C435S325000, C536S023100, C536S023200, C536S023740
Reexamination Certificate
active
10840325
ABSTRACT:
The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
REFERENCES:
patent: 4666701 (1987-05-01), Horrobin et al.
patent: 4758592 (1988-07-01), Horrobin et al.
patent: 4826877 (1989-05-01), Stewart et al.
patent: 5116871 (1992-05-01), Horrobin et al.
patent: 5443974 (1995-08-01), Hitz et al.
patent: 6136574 (2000-10-01), Knutzon et al.
patent: 6372965 (2002-04-01), Lightner et al.
patent: 6872872 (2005-03-01), Lightner et al.
patent: 6919466 (2005-07-01), Lightner et al.
patent: 2005/0266537 (2005-12-01), Chen
patent: 0005277 (1982-01-01), None
patent: WO 94/11516 (1994-05-01), None
patent: WO 03/099216 (2003-12-01), None
Supapon Passorn et al., Heterologous Expression of Mucor rouxil Δ 12-Desaturase Gene inSaccharomyces cerevisiae, Biochemical and Biophysical Research Communications, vol. 263:47-51, 1999.
Dyerberg, J. et al., Fatty Acid Composition of the plasma lipids in Greenland Eskimos, Amer. J. Clin Nutr. 28: pp. 958-966, 1975.
Dyerberg, J. et al., Eicosapentaenoic Acid and Prevention of Thrombosis and Atherosclerosis?, Lancet 2(8081): pp. 117-119, Jul. 15, 1978.
Shimokawa, H., Beneficial Effects of Eicosapentaenoic Acid on Endothelial Vasodilator Functions in Animals and Humans, World Rev. Nutr. Diet, 88: pp. 100-108, 2001.
Von Schacky et al.,Fatty Acids from Eskimos to Clical Cardiology—What Took Us So Long?, World Rev. Nutr. Diet, 88: pp. 90-99, 2001.
Domergue et al., Cloning and functional characterization ofPhaeodactylum tricomutuimfront-end desaturases involved in eicosapentaenoic acid biosynthesis, Eur. J. Biochem. 269: 4105-4113, 2002.
Beaudoin et al., Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway, Proc. Natl. Acad. Sci. U.S.A. 97(12): 6421-6, 2000.
Dyer et al.,Metabolic engineering ofSaccharomyces cerevisiaefor production of novel lipid compounds, Appl. Eniv. Micobiol., 59: pp. 224-230, 2002.
Ratledge, Microbial Oils and Fats: An Assessment of their Commercial Potential, C., Prog. Ind. Microbiol. 16: 119-206, 1982.
Brenner et al., Regulatory function of Delta6 Desaturase—Key Enzyme of Polyunsaturated Fatty Acid Synthesis, Adv. Exp. Med. Biol. 83: pp. 85-101, 1976.
Horrobin et al., Fatty acid metabolism in health and sisease: the role of delta-6-desaturase Am. J. Clin. Nutr. 57, (Suppl.) 732S-737S, 1993.
Accession No. AAG36933,Emericella nidulans, Jul. 10, 2001.
Accession No. AF110509,Mortierella alpina, Nov. 18, 1999.
Accession No. AAL13300,Mortierella alpina, Oct. 11, 2001.
Accession No. AF417244,Mortierella alpina, Oct. 11, 2001.
Accession No. AF161219,Amylomyces rouxii, Oct. 12, 1999.
Accession No. AB020033,Mortierella alpina, Jun. 26, 1999.
Accession No. AABX01000374,Neurospora crassa, Mar. 12, 2003.
Accession No. AABX01000577,Neurospora crassa, Mar. 12, 2003.
Yadav Narendra S.
Zhang Hongxiang
E. I. du Pont de Nemours and Company
Guzo David
Joike Michele
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