X'-, Y'-, Z'- axis multidimensional slit-scan flow system

Radiant energy – Luminophor irradiation – With ultraviolet source

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356318, G01N 2164

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active

043508928

ABSTRACT:
A cytofluorometer for simultaneously obtaining multidimensional slit-scan type fluorescence contours of particles, particularly biological cells, in flow. It is recognized that a cell may be effectively partitioned into orthogonal substantially planar cross sections by means of optical imaging through three slit-imaging optical systems, with each of the optical imaging systems viewing the illuminated central region of the cell. Significantly, all optical axes lie substantially in a plane perpendicular to the flow axis. As a result, the depth of focus required is only that necessary to image directly across the flow stream, rather than obliquely as in previous apparatus. This narrower depth of focus can be directly traded f

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