Wine yeast cultures

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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Details

4352536, 4352551, 4352568, 435911, C12N 100, C12N 116, C12N 120

Patent

active

061401080

ABSTRACT:
The invention provides method and compositions for yeast cultures with a predetermined desirably improved trait, such an enhanced or reduced ability to produce particular fermentation products. In one embodiment, the breeding scheme involves assembling and surveying a large population of natural wine yeast for a chosen trait; select appropriate individuals from this population and repeatedly back-crossing to well-established commercial strain by selecting HO/HO spores from the selected and commercial parental strains, treating asci with zymolase and carrying out spore-spore pairings, observing the spore pairs at intervals to detect formation of primary zygotes, and selecting and sporulating primary zygote clones; analyzing segregants of primary zygote clones and scoring for the trait; selecting the best segregants; and repeating the back-crossing procedure using a different HO/HO spore clone from the commercial parent in each cross.

REFERENCES:
Farris et al. A genetically improved wine yeast. Biotechnology Letters. Mar. 1992, vol. 14, No. 3, pp. 219-222.
Romano et al. Improvement of a Wine Saccharomyces cerevisiae Strain by a breeding Program. Applied and Environmental Microbiology. Oct. 1985, vol. 50, No. 4, pp. 1064-1067.

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