Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-08-23
1999-01-12
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 5, 435 911, 536 243, 536 2431, 536 2433, C12P 1934, C12Q 168, C07H 2102, C07H 2104
Patent
active
058587322
ABSTRACT:
The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence which may be present in a test sample. The primer sets have different sensitivities and hybridize with sub-target sequences which are different regions of the target sequence. The method generally comprises cycling a test sample suspected of containing a target sequence, an aggregate primer series, and means necessary for performing an amplification reaction; and detecting any amplified sub-target sequences. Based on a qualitative detection of the amplified sub-target sequences generated by individual primer sets, the relative quantity of the target sequence can be determined.
REFERENCES:
patent: 5213961 (1993-05-01), Bunn et al.
patent: 5219727 (1993-06-01), Wang et al.
patent: 5389512 (1995-02-01), Sninsky et al.
Quantification of Hepatitis B Virus DNA by Competitive Amplification and Hybridization on Microplates jalava, et al Biotechniques, vol. 15, No. 1, pp. 134-139, 1993.
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions Higuchi, et al Bio/Technology, vol. 11, pp. 1026-1030, 1993.
Quantification of Gene Expression over a Wide Range by the Polymerase Chain Reaction Kinoshita, et al Analytical Biochemistry, vol. 206, pp. 231-235, 1992.
Quantitation of Plasma Human Immunodeficiency Virus Type 1 RNA by Competitive Polymerase Chain Reaction Scadden, et al Journal of Infectious Diseases, vol. 165, pp. 1119-1123, 1992.
Okamoto et al, (1993), "Detection of Hepatitis C virus genome in human serum by multi-targeted polymerase chain reaction", J. Med. Virol. 41:6-10.
Matthews et al, (1988), "Analytical strategies for the use of DNA probes", Anal. Biochem. 169:1-25.
He et al, (Jul. 1994), "Primers are decisive for sensitivity of PCR", Biotechniques 17(1):82, 84, 86, 87.
Sugimoto et al, (1993), "Quantitative detection of DNA by coamplification polymerase chain reaction: A wide detectable range controlled by the thermodynamic stability of primer template duplexes", Anal. Biochem. 211:170-172.
Becker-Andre et al, (1989), "Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY)", Nucleic Acids Res. 17(22):9437-9446.
Dostal et al, (1994), "An improved method for absolute quantification of mRNA using multiplex polymerase chain reaction: determination of renin and angiotensis mRNA levels in various tissues", Anal. Biochem. 223:239-250.
Celi et al, (1994), "Determination of gene dosage by a quantitative adaption of the polymerase chain reaction (gd-PCR): Rapid detection of deletions and duplications of gene sequences", Genomics 21:304-310.
Wong et al, (1994), "Monitoring mRNA expression by polymerase chain reaction: The primer dropping method", Anal. Biochem. 223:251-258.
Foley et al, (1993), "Quantitation of RNA using the polymerase chain reaction", Trends Genet. 9(11):380-385.
Apostolakos et al, (1993), "Measurement of gene expression by multiplex competitive polymerase chain reaction", Anal. Biochem. 213:277-284.
Chelly et al, (1988), "Transcription of the dystrophin gene in human muscle and non-muscle tissues", Nature 333:858-860.
Rappolee et al, (1988), "Wound macrophages express TGF-a and other growth factors in vivo: analysis by mRNA phenotyping", Science 241:708-712.
Hetu et al, "A non-isotopic nested polymerase chain reaction method to quantitate minimal residual disease in patients with non-Hodgkin's lymphoma", Mol. Cell. Biol. 8:449-457, Dec. 1994.
Bouma Stanley R.
Solomon Natalie A.
Abbott Laboratories
Fredman Jeffrey
Yasger Paul D.
LandOfFree
Wide dynamic range nucleic acid detection using an aggregate pri does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Wide dynamic range nucleic acid detection using an aggregate pri, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Wide dynamic range nucleic acid detection using an aggregate pri will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1514379