Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2006-05-16
2006-05-16
Leary, Louise N. (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S024000, C435S029000, C435S004000, C435S968000, C435S975000
Reexamination Certificate
active
07045311
ABSTRACT:
The activity of a cell surface protease, particularly an ADAM, is determined in a rapid and sensitive assay employing a whole cell system. The assays are effective to identify effector molecules that affect the activity of a cell surface protease directly or indirectly, and to screen for therapeutic agents that modulate those effector molecules. The assays can also be used to screen for therapeutic agents that modulate the activity of a cell surface protease associated with a disease or medical condition. Kits comprising at least one component of the present assays are also provided.
REFERENCES:
patent: 4331590 (1982-05-01), Bocuslaski
patent: 4650750 (1987-03-01), Giese
patent: 4709016 (1987-11-01), Giese
patent: 4780421 (1988-10-01), Kameda
patent: 5057412 (1991-10-01), Rabin
patent: 5340716 (1994-08-01), Ullman
patent: 5360819 (1994-11-01), Giese
patent: 5470705 (1995-11-01), Grossman
patent: 5494793 (1996-02-01), Schindele
patent: 5514543 (1996-05-01), Grossman
patent: 5516636 (1996-05-01), McCapra
patent: 5516931 (1996-05-01), Giese
patent: 5536834 (1996-07-01), Singh
patent: 5565324 (1996-10-01), Still
patent: 5578498 (1996-11-01), Singh
patent: 5580732 (1996-12-01), Grossman
patent: 5602273 (1997-02-01), Giese
patent: 5604104 (1997-02-01), Giese
patent: 5610020 (1997-03-01), Giese
patent: 5616719 (1997-04-01), Davalian
patent: 5624800 (1997-04-01), Grossman
patent: 5650270 (1997-07-01), Giese
patent: 5703222 (1997-12-01), Grossman
patent: 5705622 (1998-01-01), McCapra
patent: 5709994 (1998-01-01), Pease
patent: 5721099 (1998-02-01), Still
patent: 5756726 (1998-05-01), Hemmi
patent: 5766481 (1998-06-01), Zambias
patent: 5777096 (1998-07-01), Grossman
patent: 5789172 (1998-08-01), Still
patent: 5807675 (1998-09-01), Davalian
patent: 5807682 (1998-09-01), Grossman
patent: 5843655 (1998-12-01), McGall
patent: 5843666 (1998-12-01), Akhavan-Tafti
patent: 5846839 (1998-12-01), Gallop
patent: 5849878 (1998-12-01), Cantor
patent: 5952654 (1999-09-01), Giese
patent: 5958202 (1999-09-01), Regnier
patent: 5986076 (1999-11-01), Rothschild
patent: 5989871 (1999-11-01), Grossman
patent: 6001573 (1999-12-01), Roelant
patent: 6001579 (1999-12-01), Still
patent: 6027890 (2000-02-01), Ness
patent: 6251581 (2001-06-01), Ullman
patent: 6255064 (2001-07-01), Tindal et al.
patent: 6312893 (2001-11-01), Van Ness
patent: 6322980 (2001-11-01), Singh
patent: 6331530 (2001-12-01), Breslow
patent: 6335201 (2002-01-01), Allbritton
patent: 6346384 (2002-02-01), Pollner
patent: 6346529 (2002-02-01), Floyd
patent: 6368874 (2002-04-01), Gallop
patent: 6489116 (2002-12-01), Wagner
patent: 6627400 (2003-09-01), Singh
patent: 2002/0037542 (2002-03-01), Allbritton
patent: 2003/0108978 (2003-06-01), Ciambrone et al.
patent: WO 93/06121 (1993-04-01), None
patent: WO 98/01533 (1998-01-01), None
patent: WO 98/13488 (1998-04-01), None
patent: WO 00/56925 (2000-09-01), None
patent: WO 00/66607 (2000-11-01), None
patent: WO 01/23533 (2001-04-01), None
patent: WO 01/57530 (2001-08-01), None
Hurtado et al; Neuropharmacology, V. 40(8), pp. 1094-1102, (Jun. 2001).
Bauvois, “Transmembrane proteases in focus: diversity and redundancy?”, J. Leukocyte Biology, 2001; 70:11-17.
Daub et al., “Role of transactivation of the EGF receptor in signaling by G-protein-coupled receptors”, Nature, 1996; 397:557-560.
Doedens et al., “Stimulation-induced down-regulation of Tumor Necrosis Factor-alpha converting enzyme”, J Biol Chem, 2000; 275:14598-14607.
Hooper et al., “Membrane protein secretases”, Biochem J., 1997; 321:265-279.
Howard et al., “Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types”, Biochem J., 1996; 317:45-50.
Killar et al., “Adamalysins. A family of metzincins including TNF-alpha converting enzyme (TACE)”, Ann NY Acad Sci, 1999; 878:442-452.
Lammich et al., “Constitutive and regulated α-secretase cleavage of Alzheimer's amyloid precursor protein by a disintegrin metalloprotease”,.Proc. Natl. Acad. Sci. USA, 1999; 96:3922-3927.
Luttrell et al., “Regulation of tyrosine kinase cascades by G-protein-coupled receptors”, Cur Opin Cell Bio, 1999; 11:177-183.
Prenzel, et al., “EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF”, Nature, 1999; 402:884-888.
Primakoff et al., “The ADAM gene family: surface proteins with adhesion and protease activity”, Trends Genet., 2000; 16:83-87.
Beaudet, et al., “Homogenous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen”, Genome Research, 2001, 11:600-608.
Blakely, et al., “Epidermal growth factor receptor dimerization monitored in live cells”, Nature Biotechnology, 2000, 18:218-222.
Matko, et al., “Energy Transfer Methods for Detecting for Detecting Molecular Clusters on Cell Surfaces”, Methods in Enzymology, 1997, 278:444-462.
Packard BioScience, “Principles of AlphaScreen”, Application Note ASC-001, 2001.
Price, et al., “Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology”, Methods in Molecular Biology, 2003, 218:255-267.
Sako, et al., “Single-molecule imaging of EGFR signalling on the surface of living cells”, Nature Cell Biology, 2000, 2:168-172.
Giese, “Electrophoric Release Tags: Ultrasensitive Molecular Labels Providing Multiplicity”, Trends in Analytical Chemistry, vol. 2, No. 7, 1983, pp. 166-168.
Olejnik et al., “Photocleavable Affinity Tags for Isolation and Detection of Biomolecules”, Methods in Enzymology, vol. 291, 1998, pp. 135-154.
Ullman et al., “Luminescent Oxygen Channeling Immunoassay: Measurement of Particle Binding Kinetics by Chemiluminescence”, Proc. Natl. Acad. Sci. USA, vol. 91, 1994, pp. 5426-5430.
Joppich-Kuhn et al, “Release Tags: A new class of analytical reagents,” Clin. Chem., 28: 1844-1847 (1982).
McVey et al, “Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer,” J. Biol. Chem., 276: 14092-14099 (2001).
Angers et al, “Detection of β2-Adrenergic Receptor Dimerization in Living Cells Using Bioluminescence Resonance Energy Transfer (BRET)”, PNAS, Mar. 28, 2000, vol. 97, No. 7, 3684-3689.
Luttrell et al., “Regulation of tyrosine kinase cascades by G-protein-coupled receptors”, Our Opin Cell Bio, 1999; 11:177-183.
Prenzel, et al., “EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF”, Nature, 1999; 402:884-888.
Primakoff et al., “The ADAM gene family: surface proteins with adhesion and protease activity”, Trends Genet., 2000; 16:83-87.
Beaudet, et al., “Homogenous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen”, Genome Research, 2001, 11:600-608.
Blakely, et al., “Epidermal growth factor receptor dimerization monitored in live cells”, Nature Biotechnology, 2000. 18:218-222.
Matko, et al., “Energy Transfer Methods for Detecting for Detecting Molecular Clusters on Cell Surfaces”, Methods in Enzymology, 1997, 278:444-462.
Packard BioScience, “Principles of AlphaScreen”, Application Note ASC-001, 2001.
Price, et al., “Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology”, Methods in Molecular Biology, 2003, 218-255-267.
Sako, et al., “Single-molecule imaging of EGFR signalling on the surface of living cells”, Nature Cell Biology, 2000, 2:168-172.
Giese, “Electrophoric Release Tags: Ultrasensitive Molecular Labels Providing Multiplicity”, Trends in Analytical Chemistry, vol. 2, No. 7, 1983, pp. 166-168.
Olejnik et al., “Photocleavable Affinity Tags for Isolation and Detection of Biomolecules”, Methods in Enzymology, vol. 291, 1998, pp.135-154.
Ul
Ciambrone Gary J.
Gibbons Ian
Gorthey LeeAnn
Leary Louise N.
Macevicz Stephen C.
Monogram Biosciences, Inc.
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