Whole blood immunoassay

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

Reexamination Certificate

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C436S069000, C436S522000, C435S007100, C422S073000

Reexamination Certificate

active

06878558

ABSTRACT:
A whole blood immunoassay includes the steps of mixing a whole blood sample with sensitized insoluble carrier particles to cause an immune agglutination; diluting the resulting agglutination mixture with an aqueous solution containing an erythrocyte lysing agent to lyse erythrocytes, thereby preparing an assay sample; and determining a degree of agglutination of the assay sample.

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Bester et al., “Cholate and pH reduce Interference by Sodium Dodecyl Sulfate in the Determination of DNA with Hoechst.” Analytical Biochemistry, vol. 223, No. 2, pp. 229-305, 1994.*
H.M. Shapiro, “Practical flow cytometry”, 1995, Wiley-Liss, New York XP002266284, Chapter 1.

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