Whisker-mediated transformation of embryogenic cotton...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion

Reexamination Certificate

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C800S298000, C800S314000, C435S468000, C435S470000

Reexamination Certificate

active

10391707

ABSTRACT:
Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

REFERENCES:
Sakhanokho H. et al. Crop Science, vol. 41; pp. 1235-1240.
Frame et al., 1994, Plant Journal; vol. 6; pp. 941-948.
Rajasekaran et al., Molecular Breeding, 1996 vol. 2, No. 4; pp. 307-319.

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