Wavelength-shifting probes and primers and their use in assays a

Details Wavelength-shifting probes and primers and their use in assays a Wavelength-shifting probes and primers and their use in assays a

1998-07-28

2000-03-14

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 536 221, 536 231, 536 2431, 536 2432, 536 2433, C12Q 168

Patent

active

060371307

ABSTRACT:
Hairpin-forming oligonucleotide probes and primers triple-labeled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes an increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided.

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