Wavelength-corrected spectrofluorometer

Radiant energy – Photocells; circuits and apparatus – Photocell controlled circuit

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356 97, H01J 3912

Patent

active

039671135

ABSTRACT:
A spectrofluorometer providing wavelength correction by means of an arbitrary function generator driven by a wavelength scanning element of the spectrofluorometer. The arbitrary function generator may be driven by the emission scanning monochromator to derive a wavelength-dependent voltage signal which is combined with the measure photomultiplier output to thereby provide a base-line compensated resultant output signal. Alternatively, the arbitrary function generator may be driven by the excitation scanning monochromator to derive a wavelength-dependent voltage signal which is combined with the output of a reference photomultiplier tube receiving part of the output of the excitation monochromator to derive an excitation-corrected reference signal which is in turn combined with the measure photomultiplier tube output signal to thereby provide an energy-corrected resultant output signal. An analog divider may be employed to divide this last-named resultant output signal by said excitation wavelength-dependent voltage signal to thereby derive a quantum-corrected fluorescence emission output signal. The arbitrary function generator has successive incremental wavelength range segments provided respectively with independently adjustable potentiometers to suitably shape the wavelength-dependent voltage signal curve.

REFERENCES:
patent: 3553444 (1968-01-01), Tong
patent: 3561873 (1971-02-01), Hooper
patent: 3582659 (1969-06-01), Dekker
patent: 3676005 (1972-07-01), Chance
patent: 3811781 (1974-05-01), Lowy
patent: 3854818 (1974-12-01), Bohler et al.
patent: 3883248 (1975-05-01), Rakhovsky et al.

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