Waveguide detection of right-angle-scattered light in flow cytom

Details Waveguide detection of right-angle-scattered light in flow cytom Waveguide detection of right-angle-scattered light in flow cytom

1998-02-23

2000-11-28

Font, Frank G.

Optics: measuring and testing

By particle light scattering

356338, G01N 2100

Patent

active

061542768

ABSTRACT:
A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

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