Water system virus detection

Liquid purification or separation – With alarm – indicator – register – recorder – signal or... – Responsive to fluid flow

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Details

210 96M, 210433M, B01D 1300, B01D 3100

Patent

active

041183150

ABSTRACT:
The performance of a waste-water reclamation system is monitored by introducing a non-pathogenic marker virus, bacteriophage F.sub.2, into the waste-water prior to treatment and, thereafter, testing the reclaimed water for the presence of the marker virus. A test sample is first concentrated by absorbing any marker virus onto a cellulose acetate filter in the presence of a trivalent cation at low pH and then flushing the filter with a limited quantity of a glycine buffer solution to desorb any marker virus present on the filter. Photo-optical detection of indirect passive immune agglutination by polystyrene beads indicates the performance of the water reclamation system in removing the marker virus. A closed system provides for concentrating any marker virus, initiating and monitoring the passive immune agglutination reaction, and then flushing the system to prepare for another sample. Peristaltic pumps are provided for volumetric control and for positive fluid displacement. Solenoid valves direct the output from the pumps in preselected routes to accomplish the process for concentrating and detecting the marker virus.

REFERENCES:
patent: 3127329 (1964-03-01), Andersen
patent: 3250596 (1966-05-01), Grafe
patent: 4018652 (1977-04-01), Lanham et al.
"Biological Analysis of Water and Waste Water", Millipure Manual, AM 302, pp. 63-72.

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