Water-soluble cross-linked polymer of lysyl endopeptidase, proce

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

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435 68, 435219, 435220, 435177, C12N 996, C12N 950, C12N 952, C12P 2100

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active

046346716

ABSTRACT:
A water-soluble cross-linked polymer of the enzyme lysyl endopeptidase produced by Achromobacter lyticus and a process for preparing the polymer as well as a semi-synthesis of human insulin using the polymer.

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Martinek et al, Chem. Abst., vol. 92, No. 2421m, 1980, "Role of Intramolecular Cross-Links of Different Length for Maintaining the Catalytically Active Conformation of the Enzyme".
Torchilin et al, Chem. Abst., vol. 91, No. 34911s, 1979, "Principles of Enzyme Stabilization, V. The Possibility of Enzyme Self Stabilization Under the Action of Potentially Reversible Intramolecular Cross-Linkages of Different Length".
Guire, Chem. Abst., vol. 88, No. 33848c, 1977, "Affinity Cross-Linking Agents for Enzyme Stabilization and Immobilization".
Masaki et al, Chem. Abst., vol. 95, No. 92892e, 1981, "Studies on a New Proteolytic Enzyme from Achromobacter lyticus M497-1, Specificity and Inhibition Studies of Achromobacter protease I".
Klemes et al, Biochim. Biophys. Acta, vol. 567, pp. 401-409, 1979, "Catalytic and Conformational Properties of X-Linked Derivatives of Penicillinase".

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